PDF(Pubmed)
Abstract：
Gastric cancer typically originates from the abnormal proliferation of normal cells within the gastric mucosa, eventually forming tumors. The roles of sperm-associated antigen 5 (SPAG5) and abnormal spindle-like microcephaly (ASPM) associated genes in gastric cancer are not yet clear. Gastric cancer datasets GSE51575 and GSE36076 profiles were downloaded from the GPL13607 and GPL570-generated gene expression omnibus database. The analysis included filtering for differentially expressed genes, weighted gene co-expression network analysis, functional enrichment analysis, gene set enrichment analysis, immune infiltration analysis, construction and analysis of the protein-protein interaction network, survival analysis, and Comparative Toxicogenomics Database analysis. Heatmaps of gene expression were also created. A total of 1457 differentially expressed genes were identified. According to gene ontology analysis, they are primarily enriched in the metabolic processes of organic acids, condensed chromosome centromere regions, and oxidoreductase activity. Kyoto Encyclopedia of Gene and Genome analysis showed they are mainly involved in metabolic pathways, P53 signaling pathway, and PPAR signaling pathway. The soft threshold power for weighted gene co-expression network analysis was set to 8. Three core genes (CENPE, SPAG5, and ASPM) were identified. Heatmaps of core gene expression revealed that SPAG5 and ASPM are highly expressed in gastric cancer samples and low in normal samples. Comparative Toxicogenomics Database analysis indicated that the core genes (CENPE, SPAG5, and ASPM) are associated with gastric tumors, gastric diseases, gastritis, gastric ulcers, tumors, inflammation, and necrosis. The SPAG5 and ASPM genes are overexpressed in gastric cancer tissues, and higher expression levels are associated with worse prognosis, may serve as potential prognostic markers.
摘要：
胃癌通常起源于胃粘膜内正常细胞的异常增殖，最终形成肿瘤。精子相关抗原5（SPAG5）和异常梭形小头畸形（ASPM）相关基因在胃癌中的作用尚不清楚。从GPL13607和GPL570生成的基因表达综合数据库下载胃癌数据集GSE51575和GSE36076谱。分析包括过滤差异表达的基因，加权基因共表达网络分析，功能富集分析，基因集富集分析，免疫浸润分析，蛋白质相互作用网络的构建和分析，生存分析,和比较毒性基因组学数据库分析。还创建了基因表达的热图。总共鉴定了1457个差异表达的基因。根据基因本体论分析,它们主要富含有机酸的代谢过程，浓缩染色体着丝粒区，和氧化还原酶活性。京都百科全书的基因和基因组分析显示它们主要参与代谢途径，P53信号通路,和PPAR信号通路。加权基因共表达网络分析的软阈值功率设为8。三个核心基因（CENPE，SPAG5和ASPM)被鉴定。核心基因表达的热图显示SPAG5和ASPM在胃癌样品中高表达，而在正常样品中低表达。比较毒性基因组学数据库分析表明，核心基因(CENPE，SPAG5和ASPM）与胃肿瘤有关，胃病,胃炎,胃溃疡,肿瘤，炎症,和坏死。SPAG5和ASPM基因在胃癌组织中过度表达，较高的表达水平与较差的预后相关，可能作为潜在的预后标志物。
