Abstract：
To explore the potential of diffusion kurtosis imaging (DKI) for assessing the degree of liver injury in a paracetamol-induced rat model and to simultaneously investigate the effect of intravenous gadoxetate on DKI parameters.
Paracetamol was used to induce hepatoxicity in 39 rats. The rats were pathologically classified into 3 groups: normal (n=11), mild necrosis (n=18), and moderate necrosis (n=10). DKI was performed before and, 15 min, 25 min, and 45 min after gadoxetate administration. Repeated-measures ANOVA with Tukey\'s multiple comparison test was used to investigate the effect of gadoxetate on mean diffusivity (MD) and mean diffusion kurtosis (MK) and to assess the differences in MD and MK among the three groups. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of the MD values when discriminating between the necrotic groups.
Gadoxetate had no significant effect on either the MD or the MK, and the effect size was small. The MD in the moderate necrosis group was significantly lower than that in the other two groups (F = 13.502, p < 0.001; η2 = 0.428 [95% CI: 0.082-0.637]), while the MK did not significantly differ among the three groups (F = 2.702, p = 0.081; η2 = 0.131 [95% CI: 0.001-0.4003]). The AUCs of MD for discriminating the moderate necrosis or normal group from the other groups were 0.921 (95% CI: 0.832-1.000) and 0.831 (95% CI: 0.701-0.961), respectively.
It would be better to measure the MD and MK before gadoxetate injection. MD showed potential for assessing the degree of liver necrosis in a paracetamol-induced liver injury rat model.
Paracetamol was used to induce hepatoxicity in 39 rats. The rats were pathologically classified into 3 groups: normal (n=11), mild necrosis (n=18), and moderate necrosis (n=10). DKI was performed before and, 15 min, 25 min, and 45 min after gadoxetate administration. Repeated-measures ANOVA with Tukey\'s multiple comparison test was used to investigate the effect of gadoxetate on mean diffusivity (MD) and mean diffusion kurtosis (MK) and to assess the differences in MD and MK among the three groups. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of the MD values when discriminating between the necrotic groups.
Gadoxetate had no significant effect on either the MD or the MK, and the effect size was small. The MD in the moderate necrosis group was significantly lower than that in the other two groups (F = 13.502, p < 0.001; η2 = 0.428 [95% CI: 0.082-0.637]), while the MK did not significantly differ among the three groups (F = 2.702, p = 0.081; η2 = 0.131 [95% CI: 0.001-0.4003]). The AUCs of MD for discriminating the moderate necrosis or normal group from the other groups were 0.921 (95% CI: 0.832-1.000) and 0.831 (95% CI: 0.701-0.961), respectively.
It would be better to measure the MD and MK before gadoxetate injection. MD showed potential for assessing the degree of liver necrosis in a paracetamol-induced liver injury rat model.
摘要：
目的：探讨弥散峰度成像（DKI）在对乙酰氨基酚诱导的大鼠模型中评估肝损伤程度的潜力，同时探讨静脉注射gadoxetate对DKI参数的影响。
方法：对乙酰氨基酚诱导39只大鼠肝毒性。将大鼠病理分为3组:正常(n=11),轻度坏死（n=18），中度坏死（n=10）。DKI之前进行过，15分钟,25分钟,和45分钟后gadoxetate给药。重复测量方差分析与Tukey的多重比较检验用于研究gadoxetate对平均扩散系数（MD）和平均扩散峰度（MK）的影响，并评估三组之间MD和MK的差异。进行受试者工作特征（ROC）曲线分析以评估区分坏死组时MD值的诊断准确性。
结果：加多酸酯对MD或MK均无显著影响，效果很小。中度坏死组的MD明显低于其他两组（F=13.502，p<0.001；η2=0.428[95％CI：0.082-0.637]），而MK在三组间无显著差异(F=2.702,p=0.081;η2=0.131[95%CI:0.001-0.4003])。MD用于区分中度坏死或正常组与其他组的AUC分别为0.921（95％CI：0.832-1.000）和0.831（95％CI：0.701-0.961），分别。
结论：在注射gadoxetate之前测量MD和MK会更好。MD在扑热息痛诱导的肝损伤大鼠模型中显示出评估肝坏死程度的潜力。
方法：对乙酰氨基酚诱导39只大鼠肝毒性。将大鼠病理分为3组:正常(n=11),轻度坏死（n=18），中度坏死（n=10）。DKI之前进行过，15分钟,25分钟,和45分钟后gadoxetate给药。重复测量方差分析与Tukey的多重比较检验用于研究gadoxetate对平均扩散系数（MD）和平均扩散峰度（MK）的影响，并评估三组之间MD和MK的差异。进行受试者工作特征（ROC）曲线分析以评估区分坏死组时MD值的诊断准确性。
结果：加多酸酯对MD或MK均无显著影响，效果很小。中度坏死组的MD明显低于其他两组（F=13.502，p<0.001；η2=0.428[95％CI：0.082-0.637]），而MK在三组间无显著差异(F=2.702,p=0.081;η2=0.131[95%CI:0.001-0.4003])。MD用于区分中度坏死或正常组与其他组的AUC分别为0.921（95％CI：0.832-1.000）和0.831（95％CI：0.701-0.961），分别。
结论：在注射gadoxetate之前测量MD和MK会更好。MD在扑热息痛诱导的肝损伤大鼠模型中显示出评估肝坏死程度的潜力。
