PDF(Pubmed)
Abstract：
Gallic acid is a widely recognized bioactive compound that falls under the category of secondary polyphenolic metabolites and is fairly found in mango fruit waste, specifically in mango seed kernel (MSK). This study aimed to adopt a green extraction approach to extract this valuable compound via ultrasound-assisted extraction (UAE) without using organic solvents but only water to obtain hazard-free extracts, and the cost of extraction can be minimal. pH (2-8), solvent ratio (20-60 mL/g), temperature (30-60°C) and time (30-60 min) of extraction were the independent variables used for extraction optimization. Single-factor experiments to obtain working ranges for selected extraction variables were carried out. A central composite design using response surface methodology was used to determine the optimum condition to obtain the maximum yield of gallic acid from MSK. The optimized extraction conditions were 3.9 pH, 36.25 mL/g solvent ratio, and 39.4°C of extraction temperature for 21.3 min. As a result, the optimized yield was 5.76 ± 0.41 mg/g, which was comparably equal to and/or better than the other solvent extraction systems. The results showed that gallic acid could efficiently be extracted via UAE under these optimal conditions. It is safer than extraction systems involving hazardous solvents that can be feasibly used for its nutraceutical and therapeutic applications.
摘要：
没食子酸是一种广泛认可的生物活性化合物，属于次生多酚代谢产物类别，在芒果水果废物中相当多。特别是在芒果种子内核（MSK）。本研究旨在采用绿色提取方法，通过超声辅助提取（UAE）提取这种有价值的化合物，而不使用有机溶剂，而只使用水，以获得无害的提取物。和提取的成本可以是最小的。pH（2-8），溶剂比（20-60mL/g），提取温度（30-60°C）和时间（30-60分钟）是用于提取优化的独立变量。进行了单因素实验，以获得选定提取变量的工作范围。使用响应面方法的中心复合材料设计用于确定从MSK获得没食子酸的最大产率的最佳条件。优化的提取条件为pH3.9,36.25mL/g溶剂比，和39.4°C的提取温度为21.3min。因此,优化产量为5.76±0.41mg/g，这与其他溶剂萃取系统相当地相同和/或更好。结果表明，在这些最佳条件下，通过UAE可以有效地提取没食子酸。它比涉及危险溶剂的提取系统更安全，可以合理地用于其营养和治疗应用。
