PDF(Pubmed)
Abstract：
Fusarium crown rot (FCR) is one of the most important soilborne diseases affecting wheat production. To investigate the diversity of the pathogens causing this disease, 199 diseased wheat samples were collected from 13 cities in Shandong province. In total, 468 isolates were obtained, and from these isolates, 11 Fusarium species were identified based on phylogenetic analyses with the translation elongation factor-1α (TEF-1α), RNA polymerase II largest subunit (RPB1), and RNA polymerase II second largest subunit (RPB2) gene sequences. Of these Fusarium isolates, 283 were identified as Fusarium pseudograminearum and the remaining isolates were identified as Fusarium graminearum (n = 113), Fusarium sinensis (n = 28), Fusarium acuminatum (n = 18), Fusarium incarnatum (n = 13), Fusarium ipomoeae (n = 5), Fusarium flocciferum (n = 3), Fusarium proliferatum (n = 2), Fusarium asiaticum (n = 1), Fusarium culmorum (n = 1), and Fusarium oxysporum (n = 1), suggesting that F. pseudograminearum is the dominant pathogen of FCR of wheat in Shandong province. Pathogenicity tests demonstrated that all 11 Fusarium species could cause typical symptoms of FCR on wheat seedlings. The results of the study indicate that a greater diversity of Fusarium species can cause FCR of wheat in Shandong province than that has been previously reported. This is the first report in the world of Fusarium incarnatum, Fusarium ipomoeae, and Fusarium flocciferum as pathogens causing FCR in wheat.
摘要：
镰刀菌冠腐病（FCR）是影响小麦生产的最重要的土壤传播疾病之一。为了研究引起这种疾病的病原体的多样性，从山东省13个地市收集了199份病态小麦样品。总的来说,获得468个分离株，从这些分离物中，根据翻译延伸因子-1α（TEF-1α）的系统发育分析，鉴定出11种镰刀菌，RNA聚合酶II最大亚基(RPB1),和RNA聚合酶II第二大亚基(RPB2)基因序列。在这些镰刀菌分离物中，283被鉴定为假镰刀菌，其余分离株被鉴定为镰刀菌（n=113），中华镰刀菌（n=28），尖孢镰刀菌（n=18），镰刀菌（n=13），镰刀菌（n=5），絮凝镰刀菌（n=3），镰刀菌（n=2），亚洲镰刀菌(n=1)，镰刀菌（n=1），和尖孢镰刀菌（n=1），表明假赤霉病是山东省小麦FCR的主要病原。致病性试验表明，所有11种镰刀菌都能在小麦幼苗上引起典型的FCR症状。研究结果表明，山东省小麦FCR的镰刀菌物种多样性比以前报道的要大。这是镰刀菌世界上的第一份报告，镰刀菌，和絮状镰刀菌是引起小麦FCR的病原体。
