PDF(Pubmed)
Abstract：
UNASSIGNED: The involvement of ATP and cAMP in sperm function has been extensively documented, but the understanding of the role of adenosine and adenosine receptors remains incomplete. This study aimed to examine the presence of adenosine A2A receptor (A2AR) and study the functional role of A2AR in human sperm.
UNASSIGNED: The presence and localization of A2AR in human sperm were examined by western blotting and immunofluorescence assays. The functional role of A2AR in sperm was assessed by incubating human sperm with an A2AR agonist (regadenoson) and an A2AR antagonist (SCH58261). The sperm level of A2AR was examined by western blotting in normozoospermic and asthenozoospermic men to evaluate the association of A2AR with sperm motility and in vitro fertilization (IVF) outcomes.
UNASSIGNED: A2AR with a molecular weight of 43 kDa was detected in the tail of human sperm. SCH58261 decreased the motility, penetration ability, intracellular Ca2+ concentration, and CatSper current of human sperm. Although regadenoson did not affect these sperm parameters, it alleviated the adverse effects of SCH58261 on these parameters. In addition, the mean level of A2AR in sperm from asthenozoospermic men was lower than that in sperm from normozoospermic men. The sperm level of A2AR was positively correlated with progressive motility. Furthermore, the fertilization rate during IVF was lower in men with decreased sperm level of A2AR than in men with normal sperm level of A2AR.
UNASSIGNED: These results indicate that A2AR is important for human sperm motility and is associated with IVF outcome.
UNASSIGNED: The presence and localization of A2AR in human sperm were examined by western blotting and immunofluorescence assays. The functional role of A2AR in sperm was assessed by incubating human sperm with an A2AR agonist (regadenoson) and an A2AR antagonist (SCH58261). The sperm level of A2AR was examined by western blotting in normozoospermic and asthenozoospermic men to evaluate the association of A2AR with sperm motility and in vitro fertilization (IVF) outcomes.
UNASSIGNED: A2AR with a molecular weight of 43 kDa was detected in the tail of human sperm. SCH58261 decreased the motility, penetration ability, intracellular Ca2+ concentration, and CatSper current of human sperm. Although regadenoson did not affect these sperm parameters, it alleviated the adverse effects of SCH58261 on these parameters. In addition, the mean level of A2AR in sperm from asthenozoospermic men was lower than that in sperm from normozoospermic men. The sperm level of A2AR was positively correlated with progressive motility. Furthermore, the fertilization rate during IVF was lower in men with decreased sperm level of A2AR than in men with normal sperm level of A2AR.
UNASSIGNED: These results indicate that A2AR is important for human sperm motility and is associated with IVF outcome.
摘要：
■ATP和cAMP在精子功能中的参与已被广泛记录，但是对腺苷和腺苷受体的作用的理解仍然不完整。本研究旨在检测腺苷A2A受体（A2AR）的存在，并研究A2AR在人类精子中的功能作用。
■通过蛋白质印迹和免疫荧光测定法检查了人精子中A2AR的存在和定位。通过将人精子与A2AR激动剂（regadenoson）和A2AR拮抗剂（SCH58261）孵育来评估A2AR在精子中的功能作用。通过蛋白质印迹法检查正常精子症和弱精子症男性的A2AR精子水平，以评估A2AR与精子活力和体外受精（IVF）结局的关系。
■在人精子的尾巴中检测到分子量为43kDa的A2AR。SCH58261降低了运动性，穿透能力，细胞内Ca2+浓度,和人类精子的CatSper电流。虽然regadenoson并不影响这些精子参数，它减轻了SCH58261对这些参数的不利影响。此外,弱精子症男性精子中A2AR的平均水平低于正常精子症男性精子。A2AR的精子水平与进行性运动呈正相关。此外，A2AR精子水平降低的男性IVF受精率低于A2AR精子水平正常的男性。
■这些结果表明A2AR对人类精子运动很重要，并且与IVF结局相关。
■通过蛋白质印迹和免疫荧光测定法检查了人精子中A2AR的存在和定位。通过将人精子与A2AR激动剂（regadenoson）和A2AR拮抗剂（SCH58261）孵育来评估A2AR在精子中的功能作用。通过蛋白质印迹法检查正常精子症和弱精子症男性的A2AR精子水平，以评估A2AR与精子活力和体外受精（IVF）结局的关系。
■在人精子的尾巴中检测到分子量为43kDa的A2AR。SCH58261降低了运动性，穿透能力，细胞内Ca2+浓度,和人类精子的CatSper电流。虽然regadenoson并不影响这些精子参数，它减轻了SCH58261对这些参数的不利影响。此外,弱精子症男性精子中A2AR的平均水平低于正常精子症男性精子。A2AR的精子水平与进行性运动呈正相关。此外，A2AR精子水平降低的男性IVF受精率低于A2AR精子水平正常的男性。
■这些结果表明A2AR对人类精子运动很重要，并且与IVF结局相关。
