PDF(Pubmed)
Abstract：
UNASSIGNED: Abnormal hippocampal neurodevelopment, particularly in the dentate gyrus region, may be a key mechanism of attention-deficit/hyperactivity disorder (ADHD). In this study, we investigate the effect of the most commonly used Chinese herb for the treatment of ADHD, Rehmanniae Radix Preparata (RRP), on behavior and hippocampal neurodevelopment in spontaneously hypertensive rats (SHR).
UNASSIGNED: Behavior tests, including Morris water maze (MWM) test, open field test (OFT) and elevated plus maze (EPM) test were performed to assess the effect of RRP on hyperactive and impulsive behavior. Hippocampal neurodevelopment was characterized by transmission electron microscopy, immunofluorescence, Golgi staining and Nissl staining approaches. Regulatory proteins such as Trkb, CDK5, FGF2/FGFR1 were examined by Western blot analysis.
UNASSIGNED: The results showed that RRP could effectively control the impulsive and spontaneous behavior and improve the spatial learning and memory ability. RRP significantly reduced neuronal loss and increased the number of hippocampal stem cells, and promoted synaptic plasticity. In addition, FGF/FGFR signaling was upregulated after RRP treatment.
UNASSIGNED: RRP can effectively reduce impulsive and spontaneous behavior and ameliorate hippocampal neurodevelopmental abnormalities in ADHD rat model.
UNASSIGNED: Behavior tests, including Morris water maze (MWM) test, open field test (OFT) and elevated plus maze (EPM) test were performed to assess the effect of RRP on hyperactive and impulsive behavior. Hippocampal neurodevelopment was characterized by transmission electron microscopy, immunofluorescence, Golgi staining and Nissl staining approaches. Regulatory proteins such as Trkb, CDK5, FGF2/FGFR1 were examined by Western blot analysis.
UNASSIGNED: The results showed that RRP could effectively control the impulsive and spontaneous behavior and improve the spatial learning and memory ability. RRP significantly reduced neuronal loss and increased the number of hippocampal stem cells, and promoted synaptic plasticity. In addition, FGF/FGFR signaling was upregulated after RRP treatment.
UNASSIGNED: RRP can effectively reduce impulsive and spontaneous behavior and ameliorate hippocampal neurodevelopmental abnormalities in ADHD rat model.
摘要：
■海马神经发育异常，特别是在齿状回区，可能是注意力缺陷多动障碍（ADHD）的关键机制。在这项研究中,我们调查了最常用的中草药治疗ADHD的效果，熟地黄(RRP),自发性高血压大鼠（SHR）的行为和海马神经发育。
■行为测试，包括莫里斯水迷宫（MWM）测试，进行了开放场测试（OFT）和高架迷宫（EPM）测试，以评估RRP对过度活跃和冲动行为的影响。海马神经发育的特点是通过透射电子显微镜，免疫荧光，高尔基染色和Nissl染色办法。调节蛋白如Trkb,通过蛋白质印迹分析检查CDK5、FGF2/FGFR1。
■结果表明，RRP可以有效地控制冲动和自发行为，并提高空间学习和记忆能力。RRP显著减少神经元丢失，增加海马干细胞的数量，并促进突触可塑性。此外,RRP治疗后FGF/FGFR信号上调。
■RRP能有效减轻ADHD模型大鼠的冲动和自发行为，改善海马神经发育异常。
■行为测试，包括莫里斯水迷宫（MWM）测试，进行了开放场测试（OFT）和高架迷宫（EPM）测试，以评估RRP对过度活跃和冲动行为的影响。海马神经发育的特点是通过透射电子显微镜，免疫荧光，高尔基染色和Nissl染色办法。调节蛋白如Trkb,通过蛋白质印迹分析检查CDK5、FGF2/FGFR1。
■结果表明，RRP可以有效地控制冲动和自发行为，并提高空间学习和记忆能力。RRP显著减少神经元丢失，增加海马干细胞的数量，并促进突触可塑性。此外,RRP治疗后FGF/FGFR信号上调。
■RRP能有效减轻ADHD模型大鼠的冲动和自发行为，改善海马神经发育异常。
