PDF(Pubmed)
Abstract：
UNASSIGNED: Wild rodents are key hosts for Cryptosporidium transmission, yet there is a dearth of information regarding their infection status in the Inner Mongolian Autonomous Region and Liaoning Province of China. Therefore, the present study was conducted to determine the prevalence and genetic characteristics of Cryptosporidium among wild rodents residing in these two provinces.
UNASSIGNED: A total of 486 rodents were captured, and fresh feces were collected from each rodent\'s intestine for DNA extraction. Species identification of rodents was performed through PCR amplification of the vertebrate cytochrome b (cytb) gene. To detect the presence of Cryptosporidium in all fecal samples, PCR analysis and sequencing of the partial small subunit of the ribosomal RNA (rRNA) gene were performed.
UNASSIGNED: Four species of rodents were identified: Rattus norvegicus, Mus musculus, Apodemus agrarius, and Cricetulus barabensis. Positive results for Cryptosporidium were obtained for 9.2% (18/195), 6.6% (7/106), 5.6% (5/89), and 6.3% (6/96) of these rodents, respectively, with an average infection rate of 7.4% (36/486). The identification revealed the presence of five Cryptosporidium species, C. ubiquitum (n = 8), C. occultus (n = 5), C. muris (n = 2), C. viatorum (n = 1), and C. ratti (n = 1), along with two Cryptosporidium genotypes: Rat genotype III (n = 10) and Rat genotype IV (n = 9).
UNASSIGNED: Based on the molecular evidence presented, the wild rodents investigated were concurrently infected with zoonotic (C. muris, C. occultus, C. ubiquitum and C. viatorum) as well as rodent-adapted (C. ratti and Rat genotype III and IV) species/genotypes, actively participating in the transmission of cryptosporidiosis.
UNASSIGNED: A total of 486 rodents were captured, and fresh feces were collected from each rodent\'s intestine for DNA extraction. Species identification of rodents was performed through PCR amplification of the vertebrate cytochrome b (cytb) gene. To detect the presence of Cryptosporidium in all fecal samples, PCR analysis and sequencing of the partial small subunit of the ribosomal RNA (rRNA) gene were performed.
UNASSIGNED: Four species of rodents were identified: Rattus norvegicus, Mus musculus, Apodemus agrarius, and Cricetulus barabensis. Positive results for Cryptosporidium were obtained for 9.2% (18/195), 6.6% (7/106), 5.6% (5/89), and 6.3% (6/96) of these rodents, respectively, with an average infection rate of 7.4% (36/486). The identification revealed the presence of five Cryptosporidium species, C. ubiquitum (n = 8), C. occultus (n = 5), C. muris (n = 2), C. viatorum (n = 1), and C. ratti (n = 1), along with two Cryptosporidium genotypes: Rat genotype III (n = 10) and Rat genotype IV (n = 9).
UNASSIGNED: Based on the molecular evidence presented, the wild rodents investigated were concurrently infected with zoonotic (C. muris, C. occultus, C. ubiquitum and C. viatorum) as well as rodent-adapted (C. ratti and Rat genotype III and IV) species/genotypes, actively participating in the transmission of cryptosporidiosis.
摘要：
■野生啮齿动物是隐孢子虫传播的关键宿主，然而，在中国的内蒙古自治区和辽宁省，有关其感染状况的信息却很少。因此,本研究旨在确定这两个省的野生啮齿动物中隐孢子虫的患病率和遗传特征。
■共捕获了486只啮齿动物，并从每个啮齿动物的肠道中收集新鲜粪便进行DNA提取。通过脊椎动物细胞色素b（cytb）基因的PCR扩增进行啮齿动物的物种鉴定。为了检测所有粪便样本中隐孢子虫的存在，对核糖体RNA(rRNA)基因的部分小亚基进行PCR分析和测序。
■确定了四种啮齿动物：褐家鼠，小家鼠,阿德米斯,和青蟹。隐孢子虫阳性结果为9.2％（18/195），6.6%(7/106)，5.6%（5/89），6.3%(6/96)的啮齿动物，分别,平均感染率为7.4%(36/486)。鉴定显示存在五种隐孢子虫，C.泛素(n=8)，C.occultus(n=5)，C.muris(n=2)，C.viatorum(n=1)，和C.ratti(n=1)，以及两种隐孢子虫基因型：大鼠基因型III（n=10）和大鼠基因型IV（n=9）。
■根据所提供的分子证据，被调查的野生啮齿动物同时感染了人畜共患(C.Muris,C.occultus,C.泛素和C.viatorum）以及啮齿动物适应性（C.ratti和Rat基因型III和IV)物种/基因型，积极参与隐孢子虫病的传播。
■共捕获了486只啮齿动物，并从每个啮齿动物的肠道中收集新鲜粪便进行DNA提取。通过脊椎动物细胞色素b（cytb）基因的PCR扩增进行啮齿动物的物种鉴定。为了检测所有粪便样本中隐孢子虫的存在，对核糖体RNA(rRNA)基因的部分小亚基进行PCR分析和测序。
■确定了四种啮齿动物：褐家鼠，小家鼠,阿德米斯,和青蟹。隐孢子虫阳性结果为9.2％（18/195），6.6%(7/106)，5.6%（5/89），6.3%(6/96)的啮齿动物，分别,平均感染率为7.4%(36/486)。鉴定显示存在五种隐孢子虫，C.泛素(n=8)，C.occultus(n=5)，C.muris(n=2)，C.viatorum(n=1)，和C.ratti(n=1)，以及两种隐孢子虫基因型：大鼠基因型III（n=10）和大鼠基因型IV（n=9）。
■根据所提供的分子证据，被调查的野生啮齿动物同时感染了人畜共患(C.Muris,C.occultus,C.泛素和C.viatorum）以及啮齿动物适应性（C.ratti和Rat基因型III和IV)物种/基因型，积极参与隐孢子虫病的传播。
