Abstract：
Chytridiomycosis, an infectious skin disease caused by the chytrid fungi, Batrachochytrium dendrobatidis and B. salamandrivorans, poses a significant threat to amphibian biodiversity worldwide. Antifungal bacteria found on the skin of chytrid-resistant amphibians could potentially provide defense against chytridiomycosis and lower mortality rates among resistant individuals. The Hong Kong newt (Paramesotriton hongkongensis) is native to East Asia, a region suspected to be the origin of chytrids, and has exhibited asymptomatic infection, suggesting a long-term coexistence with the chytrids. Therefore, the skin microbiota of this resistant species warrant investigation, along with other factors that can affect the microbiota. Among the 149 newts sampled in their natural habitats in Hong Kong, China, putative antifungal bacteria were found in all individuals. There were 314 amplicon sequence variants distributed over 25 genera of putative antifungal bacteria; abundant ones included Acinetobacter, Flavobacterium, and Novosphingobium spp. The skin microbiota compositions were strongly influenced by the inter-site geographical distances. Despite inter-site differences, we identified some core skin microbes across sites that could be vital to P. hongkongensis. The dominant cores included the family Comamonadaceae, family Chitinophagaceae, and class Betaproteobacteria. Moreover, habitat elevation and host sex also exhibited significant effects on skin microbiota compositions. The antifungal bacteria found on these newts offer an important resource for conservation against chytridiomycosis, such as developing probiotic treatments for susceptible species.
摘要：
衣原体病,由Chytrid真菌引起的感染性皮肤病，Batrachochytriumdendrobatidis和B.salamandrivorans，对全球两栖动物的生物多样性构成重大威胁。在耐chytrid的两栖动物的皮肤上发现的抗真菌细菌可能会提供针对chytrimycosis的防御和降低耐药个体的死亡率。香港牛顿（Paramesotritonhongkongensis）原产于东亚，一个被怀疑是食糜起源的地区，并表现出无症状感染，表明与食糜的长期共存。因此,这种抗性物种的皮肤微生物群值得调查，以及其他可能影响微生物群的因素。在香港的自然栖息地采样的149只new中，中国,在所有个体中都发现了推定的抗真菌细菌。有314个扩增子序列变体分布在25个推定的抗真菌细菌属;丰富的包括不动杆菌,黄杆菌,和Novoshingoumspp。皮肤微生物群组成受到站点间地理距离的强烈影响。尽管站点间存在差异，我们在不同部位发现了一些核心的皮肤微生物，这些微生物可能对洪孔虫至关重要。占主导地位的核心包括Coamonadaceae家族，科，和Betaproteobacteria类。此外，栖息地升高和宿主性别对皮肤微生物群组成也表现出显着影响。在这些new上发现的抗真菌细菌提供了重要的保护资源，例如开发易感物种的益生菌治疗。
