Abstract：
Multidrug resistance, severe side effects, and high cancer treatment costs are still well-known issues and remain an open challenge. These factors reduce the therapy\'s efficiency and safety, seriously affecting human health. Developing therapeutic approaches based on plant extracts, especially based on essential oils with cytotoxic and antioxidant properties, could be of efficacious strategies. This work incorporated Thymus capitatus essential oil (TEO) in liposomes. Thymus capitatus is a plant native to the northern region of Albania and found specifically in the Mediterranean region. TEO has several biological activities and cytotoxic properties. Due to its volatility, poor solubility, and chemical instability, however, its applicability is restricted. Incorporation into liposomes enables its effective use because the exposure time to the active compounds can be extended, increasing its efficacy against colorectal cancer cell lines, as highlighted in in vitro studies. TEO demonstrated detectable cytotoxic action against HT-29 colorectal cancer cells, and this action could be enhanced by applying various formulations of TEO-loaded liposomes to this cell line. Among the tested nanosystems, TEO-Phospholipon 90H liposomes showed more significant cytotoxic effects than TEO-Lipoid S100 liposomes and TEO-Phospholipon 85G liposomes. TEO-Phospholipon 90 H liposomes also maintained its physicochemical stability for six months at 25 °C. This research suggests that TEO, particularly when encapsulated in TEO-Phospholipon 90 H liposomes, may offer a promising therapeutic approach. However, these findings are based on in vitro studies and further in vivo research is needed to validate the efficacy and safety of this approach in clinical settings.
摘要：
多药耐药,严重的副作用,和高癌症治疗成本仍然是众所周知的问题，仍然是一个开放的挑战。这些因素降低了治疗的效率和安全性,严重影响人类健康。开发基于植物提取物的治疗方法，特别是基于具有细胞毒性和抗氧化特性的精油，可能是有效的策略。这项工作将胸腺capetatus精油（TEO）纳入脂质体中。Thymuscapatus是一种原产于阿尔巴尼亚北部地区的植物，特别在地中海地区发现。TEO具有多种生物活性和细胞毒性。由于其波动性，溶解性差，和化学不稳定性，然而,其适用性受到限制。掺入脂质体使其有效使用，因为暴露于活性化合物的时间可以延长，提高其对抗结直肠癌细胞系的功效，正如在体外研究中强调的那样。TEO对HT-29结直肠癌细胞表现出可检测的细胞毒性作用，并且这种作用可以通过将各种TEO负载脂质体的制剂应用于该细胞系来增强。在测试的纳米系统中，TEO-磷脂90H脂质体比TEO-脂质S100脂质体和TEO-磷脂85G脂质体显示出更显著的细胞毒性作用。TEO-Phospholipon90H脂质体还在25°C下保持了6个月的物理化学稳定性。这项研究表明TEO,特别是当封装在TEO-磷脂90H脂质体中时，可能提供一种有希望的治疗方法。然而,这些发现是基于体外研究,需要进一步的体内研究来验证这种方法在临床环境中的有效性和安全性.
