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Abstract：
BACKGROUND: Bacterial infections and the rising antimicrobial resistance pose a significant threat to public health. Pseudomonas aeruginosa produces bacteriocins like pyocins, especially S-type pyocins, which are promising for biological applications. This research focuses on clinical P. aeruginosa isolates to assess their bacteriocin production, inhibitory spectrum, chemical structure, antibacterial agents, and preservative potential.
METHODS: The identification of P. aeruginosa was conducted through both phenotypic and molecular approaches. The inhibitory spectrum and antibacterial potential of the isolates were assessed. The kinetics of antibacterial peptide production were investigated, and the activity of bacteriocin was quantified in arbitrary units (AU ml-1). Physico-chemical characterization of the antibacterial peptides was performed. Molecular weight estimation was carried out using SDS-PAGE. qRT-PCR analysis was employed to validate the expression of the selected candidate gene.
RESULTS: The antibacterial activity of P. aeruginosa was attributed to the secretion of bacteriocin compounds, which belong to the S-type pyocin family. The use of mitomycin C led to a significant 65.74% increase in pyocin production by these isolates. These S-type pyocins exhibited the ability to inhibit the growth of both Gram-negative (P. mirabilis and P. vulgaris) and Gram-positive (S. aureus, S. epidermidis, E. hirae, S. pyogenes, and S. mutans) bacteria. The molecular weight of S-type pyocin was 66 kDa, and its gene expression was confirmed through qRT-PCR.
CONCLUSIONS: These findings suggest that S-type pyocin hold significant potential as therapeutic agents against pathogenic strains. The Physico-chemical resistance of S-type pyocin underscores its potential for broad applications in the pharmaceutical, hygiene, and food industries.
METHODS: The identification of P. aeruginosa was conducted through both phenotypic and molecular approaches. The inhibitory spectrum and antibacterial potential of the isolates were assessed. The kinetics of antibacterial peptide production were investigated, and the activity of bacteriocin was quantified in arbitrary units (AU ml-1). Physico-chemical characterization of the antibacterial peptides was performed. Molecular weight estimation was carried out using SDS-PAGE. qRT-PCR analysis was employed to validate the expression of the selected candidate gene.
RESULTS: The antibacterial activity of P. aeruginosa was attributed to the secretion of bacteriocin compounds, which belong to the S-type pyocin family. The use of mitomycin C led to a significant 65.74% increase in pyocin production by these isolates. These S-type pyocins exhibited the ability to inhibit the growth of both Gram-negative (P. mirabilis and P. vulgaris) and Gram-positive (S. aureus, S. epidermidis, E. hirae, S. pyogenes, and S. mutans) bacteria. The molecular weight of S-type pyocin was 66 kDa, and its gene expression was confirmed through qRT-PCR.
CONCLUSIONS: These findings suggest that S-type pyocin hold significant potential as therapeutic agents against pathogenic strains. The Physico-chemical resistance of S-type pyocin underscores its potential for broad applications in the pharmaceutical, hygiene, and food industries.
摘要：
背景：细菌感染和抗菌素耐药性的上升对公众健康构成了重大威胁。铜绿假单胞菌产生细菌素，如脓毒素，尤其是S型绿脓菌素,有希望的生物应用。这项研究的重点是临床铜绿假单胞菌分离株，以评估其细菌素的产生，抑制谱,化学结构,抗菌剂，和防腐剂的潜力。
方法：铜绿假单胞菌的鉴定通过表型和分子方法进行。评估了分离物的抑制谱和抗菌潜力。研究了抗菌肽生产的动力学，细菌素的活性以任意单位(AUml-1)定量。进行抗菌肽的物理化学表征。使用SDS-PAGE进行分子量估计。采用qRT-PCR分析来验证所选候选基因的表达。
结果：铜绿假单胞菌的抗菌活性归因于细菌素化合物的分泌，属于S型pyocin家族。丝裂霉素C的使用导致这些分离株的pyocin产量显着增加65.74％。这些S型脓霉素表现出抑制革兰氏阴性细菌生长的能力(P.mirabilis和P.vulgaris)和革兰氏阳性(S.金黄色葡萄球菌,表皮葡萄球菌，E.Hirae,美国化脓性细菌，和变形链球菌)细菌。S型脓霉素的分子量为66kDa,并通过qRT-PCR确认其基因表达。
结论：这些发现表明S型脓霉素具有作为抗致病菌株的治疗剂的重要潜力。S型脓毒素的物理化学抗性强调了其在制药中广泛应用的潜力，卫生,和食品工业。
方法：铜绿假单胞菌的鉴定通过表型和分子方法进行。评估了分离物的抑制谱和抗菌潜力。研究了抗菌肽生产的动力学，细菌素的活性以任意单位(AUml-1)定量。进行抗菌肽的物理化学表征。使用SDS-PAGE进行分子量估计。采用qRT-PCR分析来验证所选候选基因的表达。
结果：铜绿假单胞菌的抗菌活性归因于细菌素化合物的分泌，属于S型pyocin家族。丝裂霉素C的使用导致这些分离株的pyocin产量显着增加65.74％。这些S型脓霉素表现出抑制革兰氏阴性细菌生长的能力(P.mirabilis和P.vulgaris)和革兰氏阳性(S.金黄色葡萄球菌,表皮葡萄球菌，E.Hirae,美国化脓性细菌，和变形链球菌)细菌。S型脓霉素的分子量为66kDa,并通过qRT-PCR确认其基因表达。
结论：这些发现表明S型脓霉素具有作为抗致病菌株的治疗剂的重要潜力。S型脓毒素的物理化学抗性强调了其在制药中广泛应用的潜力，卫生,和食品工业。
