PDF(Pubmed)
Abstract：
As the threat posed by antimicrobial resistance grows more crucial, the development of compounds that can replace antibiotics becomes increasingly vital. Chicken cathelicidin-2 (Cath-2) belongs to the group of Host Defense Peptides (HDPs), which could provide a feasible solution for the treatment of gastrointestinal infections in poultry. It is a small peptide produced by the heterophil granulocytes of chickens as part of the innate immune response, and its immunomodulatory activity has already been demonstrated in several cell types. In this study, the effects of Cath-2 on the intestinal immune response were examined using ileal explant cultures isolated from chicken. Regarding our results, Cath-2 displayed a potent anti-inflammatory effect as it alleviated the LTA-caused elevation of interleukin (IL)-6 and IL-2 concentrations, and that of the IFN-γ/IL-10 ratio, furthermore, it increased the concentration of IL-10, alleviating the LTA-evoked decreased level of the anti-inflammatory cytokine. Moreover, when applied alone, it elevated the concentrations of IL-6, CXCLi2, and IL-2, providing evidence of its complex immunomodulatory mechanisms. In summary, Cath-2 was able to modulate the immune response of the intestinal wall not only by reducing pro-inflammatory cytokine release, but also through immune stimulation, demonstrating that it has the ability to improve innate immunity via a complex mechanism that may make it a suitable candidate for the control of intestinal infections.
摘要：
随着抗菌素耐药性带来的威胁变得越来越重要，可以替代抗生素的化合物的开发变得越来越重要。鸡cathelicidin-2（Cath-2）属于宿主防御肽（HDP）组，为家禽胃肠道感染的治疗提供了可行的解决方案。它是由鸡的异型粒细胞产生的小肽，作为先天免疫反应的一部分，其免疫调节活性已经在几种细胞类型中得到证实。在这项研究中,使用从鸡中分离的回肠外植体培养物检查Cath-2对肠道免疫应答的影响。关于我们的结果,Cath-2表现出有效的抗炎作用，因为它减轻了LTA引起的白细胞介素（IL）-6和IL-2浓度升高，以及IFN-γ/IL-10比率，此外，它增加了IL-10的浓度，减轻了LTA引起的抗炎细胞因子水平的降低。此外，当单独应用时，它升高了IL-6，CXCLi2和IL-2的浓度，为其复杂的免疫调节机制提供了证据。总之,Cath-2不仅能够通过减少促炎细胞因子的释放来调节肠壁的免疫反应，而且通过免疫刺激，证明它具有通过复杂机制改善先天免疫的能力，这可能使其成为控制肠道感染的合适候选者。
