Abstract：
Converting CO2 into value-added chemicals still remains a grand challenge. Succinic acid has long been considered as one of the top building block chemicals. This study reported efficiently upcycling CO2 into succinic acid by combining between electrochemical and engineered Escherichia coli. In this process, the Cu-organic framework catalyst was synthesized for electrocatalytic CO2-to-ethanol conversion with high Faradaic efficiency (FE, 84.7 %) and relative purity (RP, 95 wt%). Subsequently, an engineered E. coli with efficiently assimilating CO2-derived ethanol to produce succinic acid was constructed by combining computational design and metabolic engineering, and the succinic acid titer reached 53.8 mM with the yield of 0.41 mol/mol, which is 82 % of the theoretical yield. This study effort to link the two processes of efficient ethanol synthesis by electrocatalytic CO2 and succinic acid production from CO2-derived ethanol, paving a way for the production of succinic acid and other value-added chemicals by converting CO2 into ethanol.
摘要：
将二氧化碳转化为增值化学品仍然是一个巨大的挑战。琥珀酸长期以来一直被认为是顶级的积木化学品之一。这项研究报道了通过电化学和工程大肠杆菌之间的结合将CO2有效地向上循环到琥珀酸中。在这个过程中,合成了Cu-有机骨架催化剂，用于高法拉第效率的电催化CO2转化为乙醇(FE，84.7%）和相对纯度（RP，95wt%)。随后，通过结合计算设计和代谢工程，构建了一种能有效吸收CO2衍生乙醇生产琥珀酸的工程大肠杆菌，琥珀酸滴度达到53.8mM，产量为0.41mol/mol，这是82%的理论产率。这项研究努力将电催化CO2和从CO2衍生乙醇生产琥珀酸的两种有效乙醇合成过程联系起来，通过将二氧化碳转化为乙醇，为生产琥珀酸和其他增值化学品铺平了道路。
