Abstract：
Glycans are carbohydrates present in every organism that bind to specific molecules such as lectins, a diverse group of proteins. Glycans are vital to cell proliferation and protein trafficking. In addition, embryogenesis is a critical phase in the development of marine organisms. This study investigated the effects of chilling and cryoprotective agents (CPAs) on glycans in the embryos of Stenopus hispidus. The glycan profiles of embryos of S. hispidus at the heartbeat stage were analyzed using lectin arrays. The results of analyses revealed that mannose was the most abundant glycan in the S. hispidus embryos; mannose is crucial to cell proliferation, providing the energy required for embryonic growth. Additionally, the results reveled that chilling altered the content of several glycans, including fucose and Gla-GlcNAc. Chilling may promote monosaccharide accumulation, facilitating osmotic regulation of cells and signal molecules to aid S. hispidus embryos in adapting to cold conditions. Changes were also observed in the lectins NPA, orysata, PALa, ASA, discoidin II, discoidin I, UDA, PA-IIL, and PHA-P after the samples were treated with different CPAs. DMSO may minimize cell damage during exposure to chilling by preserving cell structures, membrane properties, and functions. The present study is the first to investigate the profiles and functions of glycans in shrimp embryos subjected to low-temperature injuries. This study enhances the understanding of cell reproduction during embryogenesis and provides valuable information for the study of glycans in embryos.
摘要：
聚糖是存在于每个生物体中的碳水化合物，与特定分子如凝集素结合，一组不同的蛋白质。聚糖对细胞增殖和蛋白质运输至关重要。此外,胚胎发生是海洋生物发育的关键阶段。这项研究调查了冷冻和冷冻保护剂（CPAs）对Stenopushispidus胚胎中聚糖的影响。使用凝集素阵列分析了心跳阶段的S.hispidus胚胎的聚糖谱。分析结果表明，甘露糖是S.hispidus胚胎中最丰富的聚糖；甘露糖对细胞增殖至关重要，提供胚胎生长所需的能量。此外,结果显示，冷却改变了几种聚糖的含量，包括岩藻糖和Gla-GlcNAc.低温可促进单糖积累，促进细胞和信号分子的渗透调节，以帮助S.hispidus胚胎适应寒冷条件。在凝集素NPA中也观察到变化，orysata,帕拉,ASA,盘状蛋白II,盘状蛋白I,UDA,PA-IIL,和PHA-P后的样品用不同的CPA处理。DMSO可以通过保留细胞结构来最小化暴露于冷冻过程中的细胞损伤，膜性质，和功能。本研究是首次研究低温损伤对虾胚胎中聚糖的分布和功能。这项研究增强了对胚胎发生过程中细胞繁殖的理解，并为研究胚胎中的聚糖提供了有价值的信息。
