Abstract：
Ciprofloxacin (CIP) and levofloxacin (LEV) are broad-spectrum antibiotics with potent antibacterial activity. Although many studies have shown that antibiotics can lead to gut microbiota disruption, the effects of CIP and LEV on gut microbial colonization at the embryonic stage remain poorly characterized. Here, we evaluated the response of Bufo gargarizans embryos in terms of gut microbiota colonization, growth and developmental stages to CIP and LEV exposure. Embryos treated with 100 μg/L CIP and LEV exhibited significantly reduced diversity and richness of the gut microbiota, as well as altered community structure. Both CIP and LEV treatments resulted in an increase in the pathogenic bacteria Bosea and Aeromonas, and they appeared to be more resistant to CIP than LEV. Additionally, CIP exposure caused reduced total length and delayed the development in B. gargarizans embryos, while LEV increased the total length and promoted embryonic development. The present study revealed the adverse effects of CIP and LEV exposure on host gut microbiota, growth and development during the embryonic stage, and contributed new perspectives to the evaluation of early aquatic ecological risk under CIP and LEV exposure.
摘要：
环丙沙星（CIP）和左氧氟沙星（LEV）是具有有效抗菌活性的广谱抗生素。尽管许多研究表明抗生素可以导致肠道微生物群的破坏，CIP和LEV对胚胎期肠道微生物定植的影响尚不明确。这里,我们评估了Bufogargarizans胚胎在肠道微生物群定植方面的反应，CIP和LEV暴露的生长和发育阶段。用100μg/LCIP和LEV处理的胚胎表现出显著降低的肠道微生物群的多样性和丰富度,以及改变的社区结构。CIP和LEV处理均导致病原菌博西阿和气单胞菌增加，它们对CIP的抗性似乎比LEV更强。此外,CIP暴露会导致大芽孢杆菌胚胎的总长度缩短和发育延迟，LEV增加总长度，促进胚胎发育。本研究揭示了CIP和LEV暴露对宿主肠道微生物群的不利影响，在胚胎阶段的生长和发育，并为CIP和LEV暴露下的早期水生生态风险评估提供了新的视角。
