Abstract：
Paralytic shellfish toxins (PSTs) can pose a serious threat to human health. Among them, saxitoxin (STX) is one of the most potent natural neurotoxins. Here, the copepod Tigriopus japonicus, was exposed to environmentally relevant concentrations (2.5 and 25 μg/L) STX for 48 h. Although no lethal effects were observed at both concentrations, the transcriptome was significantly altered, and displayed a concentration-dependent response. STX exposure decreased the copepod\'s metabolism and compromised immune defense and detoxification. Additionally, STX disturbed signal transduction, which might affect other cellular processes. STX exposure could inhibit the copepod\'s chitin metabolism, disrupting its molting process. Also, the processes related to damage repair and protection were up-regulated to fight against high concentration exposure. Collectively, this study has provided an early warning of PSTs for coastal ecosystem not only because of their potent toxicity effect but also their bioaccumulation that can transfer up the food chain after ingestion by copepods.
摘要：
麻痹性贝类毒素(PST)可对人类健康构成严重威胁。其中,毒素（STX）是最有效的天然神经毒素之一。这里,棘皮科足动物,暴露于环境相关浓度（2.5和25μg/L）STX48小时。尽管在这两种浓度下都没有观察到致死作用，转录组显著改变，并显示浓度依赖性反应。STX暴露降低了co足类的新陈代谢，并损害了免疫防御和解毒。此外,STX受干扰的信号转导，这可能会影响其他细胞过程。STX暴露可以抑制co足类动物的几丁质代谢，破坏它的蜕皮过程。此外，与损伤修复和保护相关的过程被上调，以对抗高浓度暴露。总的来说，这项研究为沿海生态系统的PST提供了预警，这不仅是因为它们具有强大的毒性作用，而且还因为它们的生物积累可以在co足类摄入后向食物链转移。
