Abstract：
Wound healing involves multiple populations of cells, the extracellular matrix, and soluble mediators\' actions like growth factors and cytokines. Wound care was the target of many research, utilizing new therapy techniques and the progression of acute and chronic wound treatments with techniques involving plants to improve healing and decrease the side effects of drugs. When fenugreek is applied to an ulcer, its anti-inflammatory components are released, reducing unnecessary inflammation and accelerating the healing process. Healing is controlled by growth factors that naturally activate and boost the proliferation of cells, such as Ki-67, which is associated with the growth fraction and represents the cell\'s ability to proliferate. The current study aims to assess the expression of Ki-67 in rat mucosal ulcers treated with fenugreek leave oil. Twenty-four male Wistar albino rats of 350-450 gm weight were used. The rats were grouped as follows; normal group (normal tissue without ulcer induction), control group (tissue with surgical ulcer induction on the right side), and study group (ulcer treated with fenugreek leave oil on the left side), and had been sacrificed at 3- and 7-day healing durations. Thereafter, the tissue specimens were used for immunohistochemical analysis of Ki-67. The obtained outcomes showed that expression of Ki-67 increased in groups where ulcers were induced, with significant differences between control and study groups on the 3rd day. It was concluded that the application of fenugreek oil had an accelerating effect on the healing process of mucosal ulcers, as indicated by the elevated expression level of Ki-67.
摘要：
伤口愈合涉及多种细胞群，细胞外基质，和可溶性介质的作用，如生长因子和细胞因子。伤口护理是许多研究的目标，利用新的治疗技术和涉及植物的技术的急性和慢性伤口治疗的进展，以改善愈合和减少药物的副作用。当胡芦巴被应用于溃疡时，它的抗炎成分被释放，减少不必要的炎症和加速愈合过程。愈合是由自然激活和促进细胞增殖的生长因子控制的，例如Ki-67，它与生长分数相关，代表细胞增殖的能力。本研究旨在评估用胡芦巴叶油治疗的大鼠粘膜溃疡中Ki-67的表达。使用了24只体重为350-450克的雄性Wistar白化病大鼠。大鼠分组如下:正常组(无溃疡诱导的正常组织),对照组（右侧有手术性溃疡诱导的组织），和研究组（左侧用胡芦巴留油治疗的溃疡），并在3天和7天的愈合时间被处死。此后,组织标本用于Ki-67的免疫组织化学分析.结果表明，Ki-67的表达在引起溃疡的组中增加，在第3天，对照组和研究组之间存在显着差异。结论应用胡芦巴油对粘膜溃疡的愈合过程有加速作用,如Ki-67的表达水平升高所示。
