Abstract：
BACKGROUND: Preserving sufficient oxygen supply to the tissue is fundamental for maintaining organ function. However, our ability to identify those at risk and promptly recognize tissue hypoperfusion during abdominal surgery is limited. To address this problem, we aimed to develop a new method of perfusion monitoring that can be used during surgical procedures and aid surgeons\' decision-making.
METHODS: In this experimental porcine study, thirteen subjects were randomly assigned one organ of interest [stomach (n = 3), ascending colon (n = 3), rectum (n = 3), and spleen (n = 3)]. After baseline perfusion recordings, using high-frequency, low-dose bolus injections with weight-adjusted (0.008 mg/kg) ICG, organ-supplying arteries were manually and completely occluded leading to hypoperfusion of the target organ. Continuous organ perfusion monitoring was performed throughout the experimental conditions.
RESULTS: After manual occlusion of pre-selected organ-supplying arteries, occlusion of the peripheral arterial supply translated in an immediate decrease in oscillation signal in most organs (3/3 ventricle, 3/3 ascending colon, 3/3 rectum, 2/3 spleen). Occlusion of the central arterial supply resulted in a further decrease or complete disappearance of the oscillation curves in the ventricle (3/3), ascending colon (3/3), rectum (3/3), and spleen (1/3).
CONCLUSIONS: Continuous organ-perfusion monitoring using a high-frequency, low-dose ICG bolus regimen can detect organ hypoperfusion in real-time.
METHODS: In this experimental porcine study, thirteen subjects were randomly assigned one organ of interest [stomach (n = 3), ascending colon (n = 3), rectum (n = 3), and spleen (n = 3)]. After baseline perfusion recordings, using high-frequency, low-dose bolus injections with weight-adjusted (0.008 mg/kg) ICG, organ-supplying arteries were manually and completely occluded leading to hypoperfusion of the target organ. Continuous organ perfusion monitoring was performed throughout the experimental conditions.
RESULTS: After manual occlusion of pre-selected organ-supplying arteries, occlusion of the peripheral arterial supply translated in an immediate decrease in oscillation signal in most organs (3/3 ventricle, 3/3 ascending colon, 3/3 rectum, 2/3 spleen). Occlusion of the central arterial supply resulted in a further decrease or complete disappearance of the oscillation curves in the ventricle (3/3), ascending colon (3/3), rectum (3/3), and spleen (1/3).
CONCLUSIONS: Continuous organ-perfusion monitoring using a high-frequency, low-dose ICG bolus regimen can detect organ hypoperfusion in real-time.
摘要：
背景:保持向组织的充足的氧供应对于维持器官功能是基本的。然而,我们在腹部手术期间识别高危人群和及时识别组织灌注不足的能力有限.为了解决这个问题,我们的目标是开发一种新的灌注监测方法,该方法可在外科手术过程中使用,并帮助外科医生做出决策.
方法：在本实验猪研究中，13名受试者被随机分配一个感兴趣的器官[胃(n=3)，升结肠（n=3），直肠（n=3），和脾脏（n=3）]。基线灌注记录后,使用高频，低剂量推注与体重调整（0.008mg/kg）ICG，人工完全闭塞器官供血动脉,导致靶器官灌注不足.在整个实验条件下进行连续的器官灌注监测。
结果：手动封堵预选器官供血动脉后，外周动脉供应的闭塞转化为大多数器官的振荡信号立即降低(3/3心室，3/3升结肠，3/3直肠,2/3脾）。中央动脉供应的阻塞导致心室中的振荡曲线进一步减少或完全消失（3/3），升结肠（3/3），直肠（3/3），和脾脏（1/3）。
结论：使用高频率的连续器官灌注监测，低剂量ICG推注方案可以实时检测器官灌注不足。
方法：在本实验猪研究中，13名受试者被随机分配一个感兴趣的器官[胃(n=3)，升结肠（n=3），直肠（n=3），和脾脏（n=3）]。基线灌注记录后,使用高频，低剂量推注与体重调整（0.008mg/kg）ICG，人工完全闭塞器官供血动脉,导致靶器官灌注不足.在整个实验条件下进行连续的器官灌注监测。
结果：手动封堵预选器官供血动脉后，外周动脉供应的闭塞转化为大多数器官的振荡信号立即降低(3/3心室，3/3升结肠，3/3直肠,2/3脾）。中央动脉供应的阻塞导致心室中的振荡曲线进一步减少或完全消失（3/3），升结肠（3/3），直肠（3/3），和脾脏（1/3）。
结论：使用高频率的连续器官灌注监测，低剂量ICG推注方案可以实时检测器官灌注不足。
