PDF(Pubmed)
Abstract：
Animals and plants have developed resilience mechanisms to effectively endure and overcome physical damage and environmental challenges throughout their life span. To sustain their vitality, both animals and plants employ mechanisms to replenish damaged cells, either directly, involving the activity of adult stem cells, or indirectly, via dedifferentiation of somatic cells that are induced to revert to a stem cell state and subsequently redifferentiate. Stem cell research has been a rapidly advancing field in animal studies for many years, driven by its promising potential in human therapeutics, including tissue regeneration and drug development. A major breakthrough was the discovery of induced pluripotent stem cells (iPSCs), which are reprogrammed from somatic cells by expressing a limited set of transcription factors. This discovery enabled the generation of an unlimited supply of cells that can be differentiated into specific cell types and tissues. Equally, a keen interest in the connection between plant stem cells and regeneration has been developed in the last decade, driven by the demand to enhance plant traits such as yield, resistance to pathogens, and the opportunities provided by CRISPR/Cas-mediated gene editing. Here we discuss how knowledge of stem cell biology benefits regeneration technology, and we speculate on the creation of a universal genotype-independent iPSC system for plants to overcome regenerative recalcitrance.
摘要：
动物和植物已经开发出了恢复力机制，可以在其整个生命周期中有效地承受和克服物理损伤和环境挑战。为了维持他们的活力，动物和植物都使用机制来补充受损的细胞，要么直接,涉及成人干细胞的活动，或者间接地,通过诱导恢复到干细胞状态并随后再分化的体细胞的去分化。多年来，干细胞研究一直是动物研究中快速发展的领域，受到其在人类治疗方面有前途的潜力的驱动，包括组织再生和药物开发。一个重大突破是发现了诱导多能干细胞(iPS),通过表达一组有限的转录因子从体细胞重编程。这一发现使得能够产生可分化成特定细胞类型和组织的无限供应的细胞。同样,在过去的十年中，人们对植物干细胞与再生之间的联系产生了浓厚的兴趣，在提高植物性状如产量的需求的推动下，对病原体的抗性和CRISPR/Cas介导的基因编辑提供的机会。在这里，我们讨论干细胞生物学知识如何有益于再生技术，我们推测为植物创建了一个通用的不依赖基因型的iPS细胞系统，以克服再生的顽抗。
