PDF(Pubmed)
Abstract：
There are multiple habitats in the oral cavity with bacteria, fungi, viruses, and protozoa residing in, which together constitute the oral micro-ecosystem. These microflorae in the oral cavity primarily include saliva, supragingival dental plaque, subgingival dental plaque, submucosal plaque around implants, plaque in root canals, and plaque on the mucosal surface. The interest and knowledge of the microbiome have dynamically increased with the advancement of technology. Therefore, a reliable, feasible, and practical sampling strategy for the oral microbiome is required for the investigation. This paper introduced the sampling strategy of oral microorganisms, consisting of sample collection, transport, processing, and storage. The materials and devices involved in this study are all commonly used in clinical practice or laboratory. The feasibility and reliability of the sampling methods described in this paper have been verified by multiple studies.
摘要：
口腔中有多个细菌栖息地，真菌,病毒,和原生动物居住在，它们共同构成了口腔微生态系统。这些口腔中的微生物主要包括唾液，牙龈上牙菌斑，龈下牙菌斑，植入物周围的粘膜下斑块，牙菌斑在根管，和粘膜表面的斑块。微生物组的兴趣和知识随着技术的进步而动态地增加。因此,一个可靠的，可行,和口腔微生物组的实际采样策略是调查所必需的。本文介绍了口腔微生物的采样策略,包括样本收集，运输,processing,和存储。本研究涉及的材料和设备都是临床实践或实验室中常用的。本文所述抽样方法的可行性和可靠性已通过多个研究得到验证。
