PDF(Pubmed)
Abstract：
The foraminiferal environmental DNA (eDNA) metabarcoding based on high-throughput sequencing (HTS) is a powerful tool to unravel the hidden genetic diversity and environmental lineages. Results from the eDNA approach provided valuable insight into an unplumbed diversity of soft-bodied monothalamous foraminifers [1]. Micropaleontologists overlooked monothalamids due to their soft organic and/or finely agglutinated test, which often gets destroyed during routine morphological investigations [2]. On the other hand, some foraminifera taxonomists or studies included monothalamids (soft-shelled species) in ecological and diversity investigations ranging from deep-sea locations to coastal marine habitats [1], [3], [4]. Here, we document our metabarcoding analysis of foraminiferal diversity and abundance from the mining-affected estuaries of the Indian state of Goa. High-throughput sequencing using the Illumina platform indicated the overwhelming abundance of monothalamous foraminifers in the studied estuarine sediments. For the first time, such detailed data of the foraminiferal diversity utilizing sedimentary environmental DNA (eDNA) methods was carried out in India. The raw sequence data used for analysis is available in NCBI under the Sequence Read Archive (SRA) with the BioProjects and SRA accession number: PRJNA1040471. The presented data may be used as baseline information for eDNA-based biomonitoring and biodiversity assessment surveys from Indian marine habitats across time and space.
摘要：
基于高通量测序（HTS）的有孔虫环境DNA（eDNA）代谢编码是解开隐藏的遗传多样性和环境谱系的有力工具。eDNA方法的结果提供了有价值的见解，以了解软体性一毛有孔虫的多样性[1]。微古生物学家因其柔软的有机和/或精细的凝集测试而忽略了单匹拉米，在常规形态学研究中经常被破坏[2]。另一方面,一些有孔虫分类学家或研究包括从深海地点到沿海海洋栖息地的生态和多样性调查中的单菌（软壳物种）[1]，[3],[4].这里,我们记录了对印度果阿州受采矿影响的河口有孔虫多样性和丰度的元编码分析。使用Illumina平台进行的高通量测序表明，在所研究的河口沉积物中，一等有孔虫的数量过多。第一次,在印度，利用沉积环境DNA（eDNA）方法进行了有孔虫多样性的详细数据。用于分析的原始序列数据可在NCBI中的序列读取存档(SRA)中获得,具有BioProjects和SRA登录号:PRJNA1040471。所提供的数据可用作基于eDNA的生物监测和跨时间和空间的印度海洋栖息地的生物多样性评估调查的基线信息。
