Abstract：
The review provides an overview of recent developments and applications of capillary electromigration (CE) methods for the determination of important physicochemical parameters of various (bio)molecules and (bio)particles. These parameters include actual and limiting (absolute) ionic mobilities, effective electrophoretic mobilities, effective charges, isoelectric points, electrokinetic potentials, hydrodynamic radii, diffusion coefficients, relative molecular masses, acidity (ionization) constants, binding constants and stoichiometry of (bio)molecular complexes, changes of Gibbs free energy, enthalpy and entropy and rate constants of chemical reactions and interactions, retention factors and partition and distribution coefficients. For the determination of these parameters, the following CE methods are employed: zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography. In the individual sections, the procedures for the determination of the above parameters by the particular CE methods are described.
摘要：
该综述概述了毛细管电迁移（CE）方法的最新发展和应用，用于确定各种（生物）分子和（生物）颗粒的重要物理化学参数。这些参数包括实际和限制（绝对）离子迁移率，有效的电泳迁移率，有效收费,等电点,电动电位，流体动力学半径,扩散系数，相对分子质量,酸度（电离）常数，（生物）分子复合物的结合常数和化学计量，吉布斯自由能的变化,化学反应和相互作用的焓和熵以及速率常数，保留因子以及分配和分配系数。为了确定这些参数，采用以下CE方法：在游离溶液或筛分介质中进行区带电泳，等速电泳,等电聚焦,亲和电泳,电动色谱,和电色谱。在各个部分中，描述了通过特定CE方法确定上述参数的过程。
