PDF(Pubmed)
Abstract：
BACKGROUND: Hypertrophic scarring results from myofibroblast differentiation and persistence during wound healing. Currently no effective treatment for hypertrophic scarring exists however, autologous fat grafting has been shown to improve scar elasticity, appearance, and function. The aim of this study was to understand how paracrine factors from adipose tissues and adipose-derived stromal cells (ADSC) affect fibroblast to myofibroblast differentiation.
METHODS: The transforming growth factor-β1 (TGF-β1) induced model of myofibroblast differentiation was used to test the effect of conditioned media from adipose tissue, ADSC or lipid on the proportion of fibroblasts and myofibroblasts.
RESULTS: Adipose tissue conditioned media inhibited the differentiation of fibroblasts to myofibroblasts but this inhibition was not observed following treatment with ADSC or lipid conditioned media. Hepatocyte growth factor (HGF) was readily detected in the conditioned medium from adipose tissue but not ADSC. Cells treated with HGF, or fortinib to block HGF, demonstrated that HGF was not responsible for the inhibition of myofibroblast differentiation. Conditioned media from adipose tissue was shown to reduce the proportion of myofibroblasts when added to fibroblasts previously treated with TGF-β1, however, conditioned media treatment was unable to significantly reduce the proportion of myofibroblasts in cell populations isolated from scar tissue.
CONCLUSIONS: Cultured ADSC or adipocytes have been the focus of most studies, however, this work highlights the importance of considering whole adipose tissue to further our understanding of fat grafting. This study supports the use of autologous fat grafts for scar treatment and highlights the need for further investigation to determine the mechanism.
METHODS: The transforming growth factor-β1 (TGF-β1) induced model of myofibroblast differentiation was used to test the effect of conditioned media from adipose tissue, ADSC or lipid on the proportion of fibroblasts and myofibroblasts.
RESULTS: Adipose tissue conditioned media inhibited the differentiation of fibroblasts to myofibroblasts but this inhibition was not observed following treatment with ADSC or lipid conditioned media. Hepatocyte growth factor (HGF) was readily detected in the conditioned medium from adipose tissue but not ADSC. Cells treated with HGF, or fortinib to block HGF, demonstrated that HGF was not responsible for the inhibition of myofibroblast differentiation. Conditioned media from adipose tissue was shown to reduce the proportion of myofibroblasts when added to fibroblasts previously treated with TGF-β1, however, conditioned media treatment was unable to significantly reduce the proportion of myofibroblasts in cell populations isolated from scar tissue.
CONCLUSIONS: Cultured ADSC or adipocytes have been the focus of most studies, however, this work highlights the importance of considering whole adipose tissue to further our understanding of fat grafting. This study supports the use of autologous fat grafts for scar treatment and highlights the need for further investigation to determine the mechanism.
摘要：
背景：增生性瘢痕是由伤口愈合过程中的成肌纤维细胞分化和持续造成的。然而，目前尚无有效的肥厚性瘢痕治疗方法，自体脂肪移植已被证明可以改善瘢痕弹性，外观,和功能。这项研究的目的是了解脂肪组织和脂肪来源的基质细胞（ADSC）的旁分泌因子如何影响成纤维细胞向肌成纤维细胞分化。
方法：使用转化生长因子-β1（TGF-β1）诱导的肌成纤维细胞分化模型来测试脂肪组织条件培养基的作用，ADSC或脂质对成纤维细胞和肌成纤维细胞的比例影响。
结果:脂肪组织条件培养基抑制成纤维细胞向肌成纤维细胞的分化,但在用ADSC或脂质条件培养基处理后未观察到这种抑制。肝细胞生长因子（HGF）在脂肪组织的条件培养基中很容易检测到，而不是ADSC。用HGF处理的细胞，或福替尼阻断HGF，证明HGF不是抑制肌成纤维细胞分化的原因。来自脂肪组织的条件培养基显示，当添加到先前用TGF-β1处理的成纤维细胞中时，肌成纤维细胞的比例降低，然而，条件培养基处理不能显著降低从瘢痕组织分离的细胞群中肌成纤维细胞的比例.
结论：培养的ADSC或脂肪细胞已成为大多数研究的焦点，然而,这项工作强调了考虑整个脂肪组织对进一步了解脂肪移植的重要性。这项研究支持使用自体脂肪移植治疗瘢痕，并强调需要进一步研究以确定机制。
方法：使用转化生长因子-β1（TGF-β1）诱导的肌成纤维细胞分化模型来测试脂肪组织条件培养基的作用，ADSC或脂质对成纤维细胞和肌成纤维细胞的比例影响。
结果:脂肪组织条件培养基抑制成纤维细胞向肌成纤维细胞的分化,但在用ADSC或脂质条件培养基处理后未观察到这种抑制。肝细胞生长因子（HGF）在脂肪组织的条件培养基中很容易检测到，而不是ADSC。用HGF处理的细胞，或福替尼阻断HGF，证明HGF不是抑制肌成纤维细胞分化的原因。来自脂肪组织的条件培养基显示，当添加到先前用TGF-β1处理的成纤维细胞中时，肌成纤维细胞的比例降低，然而，条件培养基处理不能显著降低从瘢痕组织分离的细胞群中肌成纤维细胞的比例.
结论：培养的ADSC或脂肪细胞已成为大多数研究的焦点，然而,这项工作强调了考虑整个脂肪组织对进一步了解脂肪移植的重要性。这项研究支持使用自体脂肪移植治疗瘢痕，并强调需要进一步研究以确定机制。
