Abstract：
In order to study the integration of reticuloendotheliosis virus (REV) in pigeonpox virus (PPV), we collected suspected pigeonpox disease material, amplified the 4b core protein gene of PPV, the gp90 gene of REV, and the integrated sequence fragments from the end of the ORF201 segment of PPV to the beginning of the LTR of REV, and sequenced these genes. The results showed that a 4b core protein fragment of 332 bp was amplified and identified as pigeonpox virus, which was named SX/TY/LTR 01/2023. Sequence analysis showed that the pigeonpox virus isolate belonged to genotype A2, which was the closest to the domestic CVL strain, with a identity of 99.4%. A band of 1191 bp was amplified from the gp90 gene of REV, named SX/TY/PPV-REV01/2023, and sequence analysis indicated that REV belonged to genotype III. The sequence analysis showed that REV belonged to genotype III, and belonged to the same large branch as the domestic isolates JSRD0701 and LNR0801, with 99.3% identity. The integrated sequence fragment was amplified to a band of 637 bp, which determined that the REV sequence was integrated in the PPV rather than a mixed infection of the two viruses. This indicates that REV was integrated in this isolation of PPV, suggesting that pigeon farms need to prevent reticuloendotheliosis at the same time when preventing pigeonpox.
摘要：
为了研究网状内皮增生病病毒(REV)在鸽痘病毒(PPV)中的整合,我们收集了可疑的猪痘病材料,扩增了PPV的4b核心蛋白基因，REV的gp90基因,以及从PPV的ORF201片段的末端到REV的LTR的开始的整合序列片段，并对这些基因进行了测序。结果表明，扩增出332bp的4b核心蛋白片段，鉴定为鸽痘病毒，它被命名为SX/TY/LTR01/2023。序列分析表明,该鸽痘病毒分离株属于与国产CVL株最接近的A2基因型,99.4%的身份。从REV的gp90基因中扩增出1191bp的条带，命名为SX/TY/PPV-REV01/2023,序列分析表明REV属于基因型III。序列分析表明，REV属于基因型III，与国内分离株JSRD0701和LNR0801属于同一大分支，同一性为99.3%。整合的序列片段被扩增为637bp的条带，这确定REV序列整合在PPV中，而不是两种病毒的混合感染。这表明REV集成在PPV的隔离中，这表明鸽子养殖场在预防鸽子痘的同时需要预防网状内皮增生。
