关键词: Economical feasibility Gamma irradiation Gene modulation Physiological changes Sprout inhibition

Mesh : Solanum tuberosum / genetics metabolism radiation effects Plant Tubers / genetics metabolism radiation effects Gene Expression Regulation, Plant / radiation effects Food Storage / methods Gamma Rays Plant Growth Regulators / metabolism Food Irradiation / methods Abscisic Acid / metabolism Indoleacetic Acids / metabolism Plant Proteins / genetics metabolism

来  源:   DOI:10.1038/s41598-024-58949-0   PDF(Pubmed)

Abstract:
Current study is the first ever storage cum market trial of radiation processed (28 tons) of potato conducted in India at a commercial scale. The objective was to affirm the efficacy of very low dose of gamma radiation processing of potato for extended storage with retained quality and to understand the plausible mechanism at the gene modulation level for suppression of potato sprouting. Genes pertaining to abscisic acid (ABA) biosynthesis were upregulated whereas its catabolism was downregulated in irradiated potatoes. Additionally, genes related to auxin buildup were downregulated in irradiated potatoes. The change in the endogenous phytohormone contents in irradiated potato with respect to the control were found to be correlated well with the differential expression level of certain related genes. Irradiated potatoes showed retention of processing attributes including cooking and chip-making qualities, which could be attributed to the elevated expression of invertase inhibitor in these tubers. Further, quality retention in radiation treated potatoes may also be related to inhibition in the physiological changes due to sprout inhibition. Ecological and economical analysis of national and global data showed that successful adoption of radiation processing may gradually replace sprout suppressants like isopropyl N-(3-chlorophenyl) carbamate (CIPC), known to leave residue in the commodity, stabilize the wholesale annual market price, and provide a boost to the industries involved in product manufacturing.
摘要:
目前的研究是在印度以商业规模进行的辐射处理(28吨)马铃薯的首次储存和市场试验。目的是确认极低剂量的γ辐射处理马铃薯的功效,以保留质量,并在基因调节水平上了解抑制马铃薯发芽的合理机制。在辐照马铃薯中,与脱落酸(ABA)生物合成有关的基因被上调,而其分解代谢被下调。此外,与生长素积累相关的基因在辐照马铃薯中下调。发现辐照马铃薯中内源植物激素含量相对于对照的变化与某些相关基因的差异表达水平密切相关。辐照土豆显示出加工属性的保留,包括烹饪和制片质量,这可能归因于这些块茎中转化酶抑制剂的表达升高。Further,经辐射处理的马铃薯的质量保留也可能与由于发芽抑制而引起的生理变化的抑制有关。对国家和全球数据的生态和经济分析表明,成功采用辐射加工可以逐渐取代发芽抑制剂,如N-(3-氯苯基)氨基甲酸异丙酯(CIPC),已知会在商品中留下残留物,稳定批发年度市场价格,并为产品制造业提供推动。
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