PDF(Pubmed)
Abstract：
Ferroptosis is an iron-dependent cell death that was discovered recently. For beneficial microbes to establish mutualistic relationships with hosts, precisely controlled cell death in plant cells is necessary. However, whether ferroptosis is involved in the endophyte‒plant system is poorly understood. Here, we reported that endophytic Streptomyces hygroscopicus OsiSh-2, which established a sophisticated and beneficial interaction with host rice plants, caused ferroptotic cell death in rice characterized by ferroptosis- and immune-related markers. Treatments with ferroptosis inhibitors and inducers, different doses of OsiSh-2, and the siderophore synthesis-deficient mutant ΔcchH revealed that only moderate ferroptosis induced by endophytes is essential for the establishment of an optimal symbiont to enhance plant growth. Additionally, ferroptosis involved in a defence-primed state in rice, which contributed to improved resistance against rice blast disease. Overall, our study provides new insights into the mechanisms of endophyte‒plant interactions mediated by ferroptosis and suggests new directions for crop yield promotion.
摘要：
铁凋亡是最近发现的一种铁依赖性细胞死亡。为了使有益的微生物与宿主建立相互关系，在植物细胞中精确控制细胞死亡是必要的。然而,人们对发酵是否参与内生菌植物系统知之甚少。这里,我们报道了内生吸湿性链霉菌OsiSh-2，它与宿主水稻植物建立了复杂和有益的相互作用，在水稻中引起铁细胞死亡，其特征是铁细胞和免疫相关标记。用铁凋亡抑制剂和诱导剂治疗，不同剂量的OsiSh-2和铁载体合成缺陷型突变体ΔcchH表明，只有内生菌诱导的适度铁凋亡对于建立最佳共生体以增强植物生长至关重要。此外,在水稻中参与防御引发状态的铁中毒，这有助于提高对稻瘟病的抗性。总的来说,我们的研究提供了新的见解，内生菌和植物相互作用的机制介导的铁死亡，并提出新的方向促进作物产量。
