关键词: Wolfiporia cocos CAZymes genes. medicinal fungus liquid fermentation mycelial biomass pachymic acid biosynthesis

Mesh : Wolfiporia / genetics metabolism Fermentation Mycelium / growth & development metabolism genetics Biomass Triterpenes / metabolism Culture Media / chemistry Gene Expression Regulation, Fungal Fungal Proteins / genetics metabolism Squalene Monooxygenase / genetics metabolism Gene Expression

来  源:   DOI:10.1093/lambio/ovae054

Abstract:
Wolfiporia cocos, a versatile fungus acclaimed for its nutritional and therapeutic benefits in Traditional Chinese Medicine, holds immense potential for pharmaceutical and industrial applications. In this study, we aimed to optimize liquid fermentation techniques and culture medium composition to maximize mycelial biomass (MB) yield, pachymic acid (PA) concentration, and overall PA production. Additionally, we investigated the molecular basis of our findings by quantifying the expression levels of genes associated with PA and MB biosynthesis using quantitative real-time polymerase chain reaction. Under the optimized fermentation conditions, significant results were achieved, with maximum MB reaching 6.68 g l-1, PA content peaking at 1.25 mg g-1, and a total PA yield of 4.76 g l-1. Notably, among the four examined genes, squalene monooxygenase, exhibited enhanced expression at 0.06 ratio under the optimized conditions. Furthermore, within the realm of carbohydrate-active enzymes, the glycoside hydrolases 16 family displayed elevated expression levels at 21 ratios, particularly during MB production. This study enhances understanding of genetic mechanism governing MB and PA production in W. cocos, highlighting the roles of squalene monooxygenase and glycoside hydrolases 16 carbohydrate-active enzymes.
摘要:
Wolfaporiacocos,一种多才多艺的真菌,因其在中药(TCM)中的营养和治疗益处而广受赞誉,对制药和工业应用具有巨大的潜力。在这项研究中,我们旨在优化液体发酵技术和培养基组成,以最大限度地提高菌丝体生物量(MB)产量,胞嘧啶酸(PA)浓度,和整体PA生产。此外,我们通过使用定量实时聚合酶链反应(qRT-PCR)定量与PA和MB生物合成相关基因的表达水平,研究了我们发现的分子基础.在优化的发酵条件下,取得了重大成果,最大MB达到6.68gL-1,PA含量达到1.25mgg-1,总PA产量为4.76gL-1。值得注意的是,在四个被检查的基因中,角鲨烯单加氧酶(SQE),在优化条件下以0.06的比率表现出增强的表达。此外,在碳水化合物活性酶(CAZymes)领域,糖苷水解酶16(GH16)家族在21个比率下表现出升高的表达水平,特别是在MB生产过程中。这项研究增强了对控制W.cocos中MB和PA产生的遗传机制的理解,强调SQE和GH16CAZymes的作用。
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