PDF(Pubmed)
Abstract：
CONCLUSIONS: In Brassica rapa, the epigenetic modifier BraA.CLF orchestrates flowering by modulating H3K27me3 levels at the floral integrator genes FT, SOC1, and SEP3, thereby influencing their expression. CURLY LEAF (CLF) is the catalytic subunit of the plant Polycomb Repressive Complex 2 that mediates the trimethylation of histone H3 lysine 27 (H3K27me3), an epigenetic modification that leads to gene silencing. While the function of CURLY LEAF (CLF) has been extensively studied in Arabidopsis thaliana, its role in Brassica crops is barely known. In this study, we focused on the Brassica rapa homolog of CLF and found that the loss-of-function mutant braA.clf-1 exhibits an accelerated flowering together with pleiotropic phenotypic alterations compared to wild-type plants. In addition, we carried out transcriptomic and H3K27me3 genome-wide analyses to identify the genes regulated by BraA.CLF. Interestingly, we observed that several floral regulatory genes, including the B. rapa homologs of FT, SOC1 and SEP3, show reduced H3K27me3 levels and increased transcript levels compared to wild-type plants, suggesting that they are direct targets of BraA.CLF and key players in regulating flowering time in this crop. In addition, the results obtained will enhance our understanding of the epigenetic mechanisms regulating key developmental traits and will aid to increase crop yield by engineering new Brassica varieties with different flowering time requirements.
摘要：
结论：在甘蓝型油菜中，表观遗传修饰剂BraA。CLF通过调节花卉整合基因FT的H3K27me3水平来协调开花，SOC1和SEP3，从而影响它们的表达。CURLYLEAF（CLF）是植物多梳抑制复合物2的催化亚基，介导组蛋白H3赖氨酸27（H3K27me3）的三甲基化，导致基因沉默的表观遗传修饰。虽然CURLYLEAF（CLF）的功能已在拟南芥中得到广泛研究，它在芸苔属作物中的作用鲜为人知。在这项研究中,我们专注于CLF的芸苔属油菜同系物，发现功能丧失突变体braA。与野生型植物相比，clf-1表现出加速的开花以及多效性表型改变。此外,我们进行了转录组学和H3K27me3全基因组分析，以鉴定BraA调节的基因。CLF。有趣的是,我们观察到一些花卉调节基因，包括FT的B.rapa同源物，与野生型植物相比，SOC1和SEP3显示出降低的H3K27me3水平和增加的转录水平，表明他们是BraA的直接目标.CLF和调节这种作物开花时间的关键参与者。此外,获得的结果将增强我们对调节关键发育性状的表观遗传机制的理解，并将通过设计具有不同开花时间要求的芸苔新品种来帮助提高作物产量。
