Abstract：
Some dipteran flies play an important role in the transmission of pathogens such as viruses, bacteria, fungi, protozoan and metazoan parasites in humans and other animals. Despite this importance, knowledge of the prevalence and molecular characteristics of some pathogens in flies is limited, and no data are available for Türkiye. In this study, we investigated the possible vector role of muscid fly species for the transmission of Enterocytozoon bieneusi Desportes (Chytridiopsida: Enterocytozoonidae), Encephalitozoon spp., Coxiella burnetii Derrick (Legionellales: Coxiellaceae) and Thelazia spp. using polymerase chain reaction (PCR) and sequence analysis. The flies were trapped in different animal-related places and surroundings from two different geographical regions of Türkiye including Central Anatolia and Middle Black Sea. According to the morphological keys, 850 (85%), 141 (14.1%) and 6 (0.6%) of the total of 1000 fly specimens identified as Musca domestica Linnaeus (Diptera: Muscidae), Stomoxys calcitrans Linnaeus (Diptera: Muscidae) and Musca autumnalis De Geer (Diptera: Muscidae), respectively. The other species including Haematobia irritans Linnaeus (Diptera: Muscidae), Muscina stabulans Fallén (Diptera: Muscidae) and Hydrotaea ignava Harris (Diptera: Muscidae) were each represented by a single specimen. Screening of the pathogens identified E. bieneusi only in M. domestica with a prevalence of 2.4%. Sequence analyses identified three known genotypes, Type IV, BEB6 and BEB8, and one novel genotype named AEUEb of E. bieneusi in M. domestica. Coxiella burnetii was detected in M. domestica and S. calcitrans with prevalences of 2.9% and 2.8%, respectively. The one specimen of H. ignava was also positive for C. burnetii. Encephalitozoon spp. and Thelazia spp. were not found in the examined specimens. Our results contribute to the current knowledge on the vector potential of muscid flies and their possible role in the transmission dynamics of certain pathogens, especially in regions where diseases are prevalent and affect public and animal health.
摘要：
一些双翅目苍蝇在病毒等病原体的传播中起着重要作用,细菌,真菌,人类和其他动物的原生动物和后生动物寄生虫。尽管有这种重要性,对苍蝇中某些病原体的流行和分子特征的了解有限，没有关于Türkiye的数据.在这项研究中,我们调查了麝香蝇物种在传播肠孢子虫（Chytridiopsida：肠孢子虫科）中可能的媒介作用，头孢菌素属。,柯西氏菌（军团菌：柯西氏菌科）和Thelaziaspp。使用聚合酶链反应（PCR）和序列分析。苍蝇被困在蒂尔基耶的两个不同地理区域，包括安纳托利亚中部和黑海中部，与动物有关的不同地方和周围环境中。根据形态学键，850（85%），在总共1000个苍蝇标本中，有141个（14.1％）和6个（0.6％）被鉴定为MuscadomesticaLinnaeus（双翅目：Muscidae），StomoxysCalcitransLinnaeus（双翅目：Muscidae）和MuscaautumnalisDeGeer（双翅目：Muscidae），分别。其他物种包括厌食症Linnaeus（双翅目：Muscidae），MuscinastabulansFallén（双翅目：Muscidae）和HydrotaeaignavaHarris（双翅目：Muscidae）分别由一个标本代表。病原体的筛选仅在家畜中鉴定出E.bieneusi，患病率为2.4％。序列分析确定了三种已知的基因型，IV型,BEB6和BEB8，以及一种新的基因型，命名为家蝇E.bieneusi的AEUEb。在家畜M.domestica和S.calcitrans中检测到伯氏柯西氏菌，患病率分别为2.9％和2.8％，分别。伊格纳瓦的一个标本也为C.burnetii阳性。头孢菌素属。和Thelaziaspp.在检查的标本中没有发现。我们的研究结果有助于了解目前有关麝香蝇的媒介潜力及其在某些病原体传播动力学中的可能作用，特别是在疾病流行并影响公众和动物健康的地区。
