Abstract：
OBJECTIVE: Apical periodontitis (AP) is the chronic inflammation of the periradicular tissues in response to root canal infection. Whilst AP has been linked with systemic inflammation and noncommunicable diseases, its potential association with nonalcoholic fatty liver disease (NAFLD) is unknown. We aimed to evaluate the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels as surrogate markers of hepatic injury, and the systemic inflammatory burden in otherwise healthy individuals with and without AP diagnosis.
METHODS: Cross-sectional study. Individuals with AP (n = 30) and healthy controls (n = 29) were recruited. The number, mean diameter (mm) and periapical index of the apical lesions of endodontic origin (ALEO) were assessed. ALT and AST levels (pg/mL) were measured through enzyme-linked immunosorbent assays. The serum levels of TNF-α, IL-4, IL-9, IL-10, IL-17A and IL-22 were evaluated by Multiplex assay. Inferential analysis was performed using t-test or Mann-Whitney tests according to data distribution and linear regression models. Data were analysed with StataV16 (p < .05).
RESULTS: ALT and AST levels were significantly higher in individuals with AP compared to controls (p < .05). Serum inflammatory biomarkers showed no significant differences between the study groups. Bivariate and multivariate analyses confirmed that AP diagnosis was independently associated with ALT and AST elevations (p < .05). Additionally, the number of ALEO positively influenced AST levels (p = .002). IL-22 on the other hand, was associated with reduced ALT levels (p = .043).
CONCLUSIONS: AP is associated with higher serum hepatic transaminases ALT and AST, potentially contributing to NAFLD physiopathology in young adults.
METHODS: Cross-sectional study. Individuals with AP (n = 30) and healthy controls (n = 29) were recruited. The number, mean diameter (mm) and periapical index of the apical lesions of endodontic origin (ALEO) were assessed. ALT and AST levels (pg/mL) were measured through enzyme-linked immunosorbent assays. The serum levels of TNF-α, IL-4, IL-9, IL-10, IL-17A and IL-22 were evaluated by Multiplex assay. Inferential analysis was performed using t-test or Mann-Whitney tests according to data distribution and linear regression models. Data were analysed with StataV16 (p < .05).
RESULTS: ALT and AST levels were significantly higher in individuals with AP compared to controls (p < .05). Serum inflammatory biomarkers showed no significant differences between the study groups. Bivariate and multivariate analyses confirmed that AP diagnosis was independently associated with ALT and AST elevations (p < .05). Additionally, the number of ALEO positively influenced AST levels (p = .002). IL-22 on the other hand, was associated with reduced ALT levels (p = .043).
CONCLUSIONS: AP is associated with higher serum hepatic transaminases ALT and AST, potentially contributing to NAFLD physiopathology in young adults.
摘要：
目的：根尖周炎（AP）是根管感染引起的根尖周组织的慢性炎症。虽然AP与全身性炎症和非传染性疾病有关，其与非酒精性脂肪性肝病(NAFLD)的潜在关联尚不清楚.我们旨在评估血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平作为肝损伤的替代标志物，以及有和没有AP诊断的健康个体的全身炎症负担。
方法：横断面研究。招募患有AP的个体(n=30)和健康对照(n=29)。数字,评估了牙髓起源（ALEO）的根尖病变的平均直径（mm）和根尖指数。通过酶联免疫吸附测定测量ALT和AST水平(pg/mL)。血清TNF-α水平,通过多重测定评价IL-4、IL-9、IL-10、IL-17A和IL-22。根据数据分布和线性回归模型，采用t检验或Mann-Whitney检验进行推断分析。用StataV16分析数据(p<0.05)。
结果：与对照组相比，AP患者的ALT和AST水平明显更高（p<0.05）。血清炎性生物标志物显示研究组之间没有显著差异。双变量和多变量分析证实，AP诊断与ALT和AST升高独立相关（p<0.05）。此外,ALEO的数量正影响AST水平（p=0.002）。另一方面，IL-22与ALT水平降低相关（p=.043）。
结论：AP与较高的血清肝转氨酶ALT和AST相关，可能导致年轻人的NAFLD生理病理。
方法：横断面研究。招募患有AP的个体(n=30)和健康对照(n=29)。数字,评估了牙髓起源（ALEO）的根尖病变的平均直径（mm）和根尖指数。通过酶联免疫吸附测定测量ALT和AST水平(pg/mL)。血清TNF-α水平,通过多重测定评价IL-4、IL-9、IL-10、IL-17A和IL-22。根据数据分布和线性回归模型，采用t检验或Mann-Whitney检验进行推断分析。用StataV16分析数据(p<0.05)。
结果：与对照组相比，AP患者的ALT和AST水平明显更高（p<0.05）。血清炎性生物标志物显示研究组之间没有显著差异。双变量和多变量分析证实，AP诊断与ALT和AST升高独立相关（p<0.05）。此外,ALEO的数量正影响AST水平（p=0.002）。另一方面，IL-22与ALT水平降低相关（p=.043）。
结论：AP与较高的血清肝转氨酶ALT和AST相关，可能导致年轻人的NAFLD生理病理。
