PDF(Pubmed)
Abstract：
With the comprehensive understanding of microorganisms and the rapid advances of physiochemical engineering and bioengineering technologies, scientists are advancing rationally-engineered bacteria as emerging drugs for treating various diseases in clinical disease management. Engineered bacteria specifically refer to advanced physiochemical or genetic technologies in combination with cutting edge nanotechnology or physical technologies, which have been validated to play significant roles in lysing tumors, regulating immunity, influencing the metabolic pathways, etc. However, there has no specific reviews that concurrently cover physiochemically- and genetically-engineered bacteria and their derivatives yet, let alone their distinctive design principles and various functions and applications. Herein, the applications of physiochemically and genetically-engineered bacteria, and classify and discuss significant breakthroughs with an emphasis on their specific design principles and engineering methods objective to different specific uses and diseases beyond cancer is described. The combined strategies for developing in vivo biotherapeutic agents based on these physiochemically- and genetically-engineered bacteria or bacterial derivatives, and elucidated how they repress cancer and other diseases is also underlined. Additionally, the challenges faced by clinical translation and the future development directions are discussed. This review is expected to provide an overall impression on physiochemically- and genetically-engineered bacteria and enlighten more researchers.
摘要：
随着对微生物的全面认识以及理化工程和生物工程技术的快速发展，在临床疾病管理中，科学家们正在推进合理工程的细菌作为治疗各种疾病的新兴药物。工程细菌特别是指先进的物理化学或遗传技术与尖端纳米技术或物理技术相结合，它们已经被证实在裂解肿瘤中起着重要的作用，调节免疫力，影响代谢途径，等。然而,目前还没有具体的评论同时涵盖物理化学和基因工程细菌及其衍生物，更不用说它们独特的设计原则以及各种功能和应用了。在这里，物理化学和基因工程细菌的应用，并对重大突破进行分类和讨论，重点是针对癌症以外的不同特定用途和疾病的特定设计原则和工程方法。基于这些物理化学和基因工程细菌或细菌衍生物开发体内生物治疗剂的组合策略，并阐明了它们如何抑制癌症和其他疾病。此外,讨论了临床翻译面临的挑战和未来的发展方向。这篇综述有望为物理化学和基因工程细菌提供总体印象，并启发更多的研究人员。
