PDF(Pubmed)
Abstract：
UNASSIGNED: Penile squamous cell carcinoma (SCC) is an extremely rare malignancy. It is usually caused by chronic human papillomavirus (HPV) 16 and HPV 18 infections. This study was conducted to investigate the immunohistochemical overexpression of p16, a surrogate marker for HPV, and to evaluate its usefulness as a potential diagnostic biomarker.
UNASSIGNED: In this cross-sectional prospective and retrospective cohort study, 56 penile squamous cell carcinoma (SCC) specimens and five penile premalignant specimens were evaluated in Kasturba Medical College, Mangalore, India, from January 2013- December 2018 in terms of clinical and histopathological features. Immunohistochemical expression for p16 in cases and controls was evaluated. Statistical comparison of p16 expression among clinical features, histological subtype, grade, and stages of tumor were done.
UNASSIGNED: Analysis of the pattern of p16 staining showed diffuse and strong nuclear and cytoplasmic expression in 32.8% of the cases. There was a highly significant association (P<0.001) of pattern of p16 expression among the HPV and non-HPV subtypes of penile carcinoma. p16 expression was not significantly associated with other prognostic parameters like site of the lesion, lymphovascular invasion, perineural invasion, histologic grade, and pathologic stage.
UNASSIGNED: Expression of p16 would be a useful tool in differentiation between the HPV-associated and non-HPV-associated subtypes of penile SCC that may be helpful in prediction of aggressiveness and invasive potential of the respective histologic subtypes.
UNASSIGNED: In this cross-sectional prospective and retrospective cohort study, 56 penile squamous cell carcinoma (SCC) specimens and five penile premalignant specimens were evaluated in Kasturba Medical College, Mangalore, India, from January 2013- December 2018 in terms of clinical and histopathological features. Immunohistochemical expression for p16 in cases and controls was evaluated. Statistical comparison of p16 expression among clinical features, histological subtype, grade, and stages of tumor were done.
UNASSIGNED: Analysis of the pattern of p16 staining showed diffuse and strong nuclear and cytoplasmic expression in 32.8% of the cases. There was a highly significant association (P<0.001) of pattern of p16 expression among the HPV and non-HPV subtypes of penile carcinoma. p16 expression was not significantly associated with other prognostic parameters like site of the lesion, lymphovascular invasion, perineural invasion, histologic grade, and pathologic stage.
UNASSIGNED: Expression of p16 would be a useful tool in differentiation between the HPV-associated and non-HPV-associated subtypes of penile SCC that may be helpful in prediction of aggressiveness and invasive potential of the respective histologic subtypes.
摘要：
■阴茎鳞状细胞癌（SCC）是一种极为罕见的恶性肿瘤。它通常是由慢性人乳头瘤病毒（HPV）16和HPV18感染引起的。这项研究是为了研究p16的免疫组织化学过度表达，p16是HPV的替代标记，并评估其作为潜在诊断生物标志物的有效性。
■在这项横断面前瞻性和回顾性队列研究中，在Kasturba医学院评估了56例阴茎鳞状细胞癌（SCC）标本和5例阴茎癌前病变标本，芒格洛尔,印度,从2013年1月至2018年12月的临床和组织病理学特征来看。评估病例和对照中p16的免疫组织化学表达。临床特征之间p16表达的统计比较，组织学亚型，grade,完成了肿瘤的分期。
■p16染色模式的分析显示，在32.8％的病例中，弥漫性和强烈的细胞核和细胞质表达。在阴茎癌的HPV和非HPV亚型中，p16的表达模式存在高度显着相关性（P<0.001）。p16表达与其他预后参数如病变部位无显著相关，淋巴管浸润,神经周浸润,组织学分级,病理阶段。
p16的表达将是区分阴茎SCC的HPV相关和非HPV相关亚型的有用工具,其可能有助于预测相应组织学亚型的侵袭性和侵袭潜力。
■在这项横断面前瞻性和回顾性队列研究中，在Kasturba医学院评估了56例阴茎鳞状细胞癌（SCC）标本和5例阴茎癌前病变标本，芒格洛尔,印度,从2013年1月至2018年12月的临床和组织病理学特征来看。评估病例和对照中p16的免疫组织化学表达。临床特征之间p16表达的统计比较，组织学亚型，grade,完成了肿瘤的分期。
■p16染色模式的分析显示，在32.8％的病例中，弥漫性和强烈的细胞核和细胞质表达。在阴茎癌的HPV和非HPV亚型中，p16的表达模式存在高度显着相关性（P<0.001）。p16表达与其他预后参数如病变部位无显著相关，淋巴管浸润,神经周浸润,组织学分级,病理阶段。
p16的表达将是区分阴茎SCC的HPV相关和非HPV相关亚型的有用工具,其可能有助于预测相应组织学亚型的侵袭性和侵袭潜力。
