PDF(Pubmed)
Abstract：
Tobacco (Nicotiana tabacum L.) is a major cash crop, and soil quality played a significant role in the yield and quality of tobacco. Most farmers cultivate tobacco in rotation with other crops to improve the soil characteristics. However, the effects of different previous crops on the soil\'s nutrient status and bacterial community for tobacco cultivation still need to be determined. Three treatments were assessed in this study, i.e., tobacco-planting soil without treatment (CK), soil with barley previously cultivated (T1), and soil with rapeseed previously cultivated (T2). The soil physical and chemical properties and the 16S rRNA gene sequence diversity of the bacterial community were analyzed. The effects of different crops on the physical and chemical properties of tobacco-planting soil and the diversity and richness of the bacterial community were comprehensively discussed. The results of this study showed that different previously cultivated crops altered the nutrient status of the soil, with changes in the ratio of NH4 +-N to NO3 --N having the most significant impact on tobacco. In CK, the ratio of NH4 +-N to NO3 --N was 1:24.2, T1-1:9.59, and T2-1:11.10. The composition of the bacterial community in tobacco-planting soil varied significantly depending on the previously cultivated crops. The richness and diversity of the bacterial community with different crops were considerably higher than without prior cultivation of different crops. The dominant bacteria in different treatments were Actinobacteriota, Proteobacteria, and Chloroflexi with their relative abundance differed. In conclusion, our study revealed significant differences in nutrient status, bacterial community diversity, and the richness of tobacco-planting soil after the preceding cultivation of different crops. Suitable crops should be selected to be previously cultivated in tobacco crop rotations in near future for sustainable agriculture.
摘要：
烟草(NicotianatabacumL.)是一种主要的经济作物，土壤质量对烟叶的产量和质量有显著影响。大多数农民与其他作物轮作种植烟草，以改善土壤特性。然而,不同前期作物对土壤养分状况和烟草栽培细菌群落的影响仍有待确定。在这项研究中评估了三种治疗方法，即，未经处理的植烟土壤（CK），先前栽培的大麦土壤（T1），和先前栽培的油菜籽土壤（T2）。剖析了泥土理化性质和细菌群落16SrRNA基因序列多样性。综合探讨了不同作物对植烟土壤理化性质及细菌群落多样性和丰富度的影响。这项研究的结果表明，不同的以前种植的作物改变了土壤的营养状况，NH4-N与NO3-N的比例变化对烟草的影响最大。在CK,NH4-N与NO3-N的比例为1：24.2，T1-1：9.59和T2-1：11.10。种植烟草的土壤中细菌群落的组成因先前种植的作物而异。与以前没有种植不同作物的细菌群落的丰富度和多样性相比，具有不同作物的细菌群落的丰富度和多样性要高得多。不同处理方式的优势菌为放线菌,变形杆菌,和氯氟菌的相对丰度不同。总之,我们的研究揭示了营养状况的显著差异，细菌群落多样性，以及先前种植不同作物后植烟土壤的丰富度。为了可持续农业，应选择合适的作物，以便在不久的将来在烟草作物轮作中预先种植。
