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Abstract：
BACKGROUND: This study investigates the protective effects of etomidate against oxidative damage in an experimental model of ovarian ischemia-reperfusion injury.
METHODS: A total of 24 female rats were randomized into three groups. Group 1 served as the control. Group 2 underwent an ovarian torsion/detorsion procedure. Group 3 underwent similar procedures as Group 2; additionally, 4 mg/kg of etomidate was administered intraperitoneally 30 minutes before ovarian detorsion. Blood samples were analyzed for lipid peroxidation, pro-inflammatory cytokine levels, and antioxidant enzyme activity RESULTS: Biochemical analysis of blood samples revealed reductions in pro-inflammatory cytokines, including interleukin-1 Beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), in Group 3 compared to Group 2 (p=0.005, p=0.016, and p<0.001, respectively). Additionally, a decrease in malondialdehyde (MDA) levels was observed in Group 3 compared to Group 2 (p<0.001). In contrast, activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were significantly increased in Group 3 compared to Group 2 (p=0.031 and p=0.001, respectively). Furthermore, Group 3 demonstrated notable reductions in histopathological scores for follicular degeneration, vascular occlusion, bleeding, and inflammation compared to Group 2 (p<0.001, p<0.001, p<0.001, and p=0.001, respectively).
CONCLUSIONS: Etomidate alleviates ischemia-reperfusion injury in a rat ovarian torsion-detorsion model by improving both histopathological and biochemical outcomes.
METHODS: A total of 24 female rats were randomized into three groups. Group 1 served as the control. Group 2 underwent an ovarian torsion/detorsion procedure. Group 3 underwent similar procedures as Group 2; additionally, 4 mg/kg of etomidate was administered intraperitoneally 30 minutes before ovarian detorsion. Blood samples were analyzed for lipid peroxidation, pro-inflammatory cytokine levels, and antioxidant enzyme activity RESULTS: Biochemical analysis of blood samples revealed reductions in pro-inflammatory cytokines, including interleukin-1 Beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), in Group 3 compared to Group 2 (p=0.005, p=0.016, and p<0.001, respectively). Additionally, a decrease in malondialdehyde (MDA) levels was observed in Group 3 compared to Group 2 (p<0.001). In contrast, activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were significantly increased in Group 3 compared to Group 2 (p=0.031 and p=0.001, respectively). Furthermore, Group 3 demonstrated notable reductions in histopathological scores for follicular degeneration, vascular occlusion, bleeding, and inflammation compared to Group 2 (p<0.001, p<0.001, p<0.001, and p=0.001, respectively).
CONCLUSIONS: Etomidate alleviates ischemia-reperfusion injury in a rat ovarian torsion-detorsion model by improving both histopathological and biochemical outcomes.
摘要：
背景：本研究探讨依托咪酯在卵巢缺血再灌注损伤实验模型中对氧化损伤的保护作用。
方法：将24只雌性大鼠随机分为3组。第1组作为对照。第2组接受了卵巢扭转/扭转手术。第3组接受了与第2组相似的程序；此外，4mg/kg依托咪酯在卵巢摘除前30分钟腹腔给药。血液样本进行脂质过氧化分析，促炎细胞因子水平，和抗氧化酶活性结果：血液样本的生化分析显示促炎细胞因子减少，包括白细胞介素-1β（IL-1β），白细胞介素-6（IL-6），和肿瘤坏死因子-α（TNF-α），与第2组相比，第3组（分别为p=0.005，p=0.016和p<0.001）。此外,与第2组相比,第3组观察到丙二醛(MDA)水平降低(p<0.001)。相比之下,抗氧化酶的活性，包括超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX），与第2组相比，第3组显着增加（分别为p=0.031和p=0.001）。此外，第3组显示卵泡变性的组织病理学评分显着降低，血管闭塞,出血,和炎症相比于第2组(分别为p<0.001、p<0.001、p<0.001和p=0.001)。
结论：依托咪酯通过改善组织病理学和生化结果减轻大鼠卵巢扭转-扭曲模型的缺血-再灌注损伤。
方法：将24只雌性大鼠随机分为3组。第1组作为对照。第2组接受了卵巢扭转/扭转手术。第3组接受了与第2组相似的程序；此外，4mg/kg依托咪酯在卵巢摘除前30分钟腹腔给药。血液样本进行脂质过氧化分析，促炎细胞因子水平，和抗氧化酶活性结果：血液样本的生化分析显示促炎细胞因子减少，包括白细胞介素-1β（IL-1β），白细胞介素-6（IL-6），和肿瘤坏死因子-α（TNF-α），与第2组相比，第3组（分别为p=0.005，p=0.016和p<0.001）。此外,与第2组相比,第3组观察到丙二醛(MDA)水平降低(p<0.001)。相比之下,抗氧化酶的活性，包括超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX），与第2组相比，第3组显着增加（分别为p=0.031和p=0.001）。此外，第3组显示卵泡变性的组织病理学评分显着降低，血管闭塞,出血,和炎症相比于第2组(分别为p<0.001、p<0.001、p<0.001和p=0.001)。
结论：依托咪酯通过改善组织病理学和生化结果减轻大鼠卵巢扭转-扭曲模型的缺血-再灌注损伤。
