Abstract：
The glycomic analysis holds significant appeal due to the diverse roles that glycans and glycoconjugates play, acting as modulators and mediators in cellular interactions, cell/organism structure, drugs, energy sources, glyconanomaterials, and more. The glycomic analysis relies on liquid-phase separation technologies for molecular purification, separation, and identification. As a miniaturized form of liquid-phase separation technology, microscale separation technologies offer various advantages such as environmental friendliness, high resolution, sensitivity, fast speed, and integration capabilities. For glycan analysis, microscale separation technologies are continuously evolving to address the increasing challenges in their unique manners. This review discusses the fundamentals and applications of microscale separation technologies for glycomic analysis. It covers liquid-phase separation technologies operating at scales generally less than 100 µm, including capillary electrophoresis, nanoflow liquid chromatography, and microchip electrophoresis. We will provide a brief overview of glycomic analysis and describe new strategies in microscale separation and their applications in glycan analysis from 2014 to 2023.
摘要：
由于聚糖和糖缀合物发挥的不同作用，糖漫画分析具有重要的吸引力，在细胞相互作用中充当调节剂和介质，细胞/有机体结构，毒品,能源,糖原材料，还有更多.糖组学分析依赖于液相分离技术进行分子纯化，分离,和识别。作为一种小型化的液相分离技术,微尺度分离技术提供各种优点，如环境友好，高分辨率,灵敏度,速度快,和集成能力。对于聚糖分析，微型分离技术正在不断发展，以其独特的方式来应对日益严峻的挑战。这篇综述讨论了用于糖色分析的微尺度分离技术的基本原理和应用。它涵盖了通常在小于100μm的规模下运行的液相分离技术，包括毛细管电泳,nanoflow液相色谱，和微芯片电泳。我们将简要概述糖素分析，并描述微尺度分离的新策略及其在2014年至2023年的聚糖分析中的应用。
