PDF(Pubmed)
Abstract：
Hyperuricemia (HUA) is a metabolic disorder caused by excessive production of uric acid (UA) or impaired uric acid metabolism. Smilax China L. has a wide range of pharmacological activities such as immunomodulatory, anti-inflammatory, and antioxidant. Its roots and rhizomes have been widely used for the treatment of HUA. However, its mechanisms for treating HUA and reducing renal impairment have not been fully elucidated. In the present study, we evaluated the effect of Smilax China L. extract (SC) on UA metabolism and further explored its mechanism of action by feeding a high-calcium and high-protein diet to chickens to induce a model of HUA in chickens. SC significantly reduced serum UA levels and improved renal function in hyperuricemic chickens. Meanwhile, SC was able to inhibit the activity of xanthine oxidase (XOD) in vivo and in vitro, reducing the production of uric acid. In addition, SC was able to increase the expression of Breast Cancer Resistance Protein (BCRP) in the kidney and ileum and increase uric acid excretion. Therefore, our results suggest that SC may be a candidate for anti-hyperuricemia.
摘要：
高尿酸血症（HUA）是由尿酸（UA）的过量产生或尿酸代谢受损引起的代谢紊乱。SmilaxChinaL.具有广泛的药理活性，如免疫调节，抗炎,和抗氧化剂。它的根和根茎已被广泛用于HUA的治疗。然而,其治疗HUA和减少肾损害的机制尚未完全阐明。在本研究中,通过饲喂高钙高蛋白日粮，建立鸡HUA模型，评价了SmilaxChinaL.提取物（SC）对UA代谢的影响，并进一步探讨了其作用机理。SC显着降低高尿酸血症鸡的血清UA水平并改善肾功能。同时,SC能够在体内和体外抑制黄嘌呤氧化酶（XOD）的活性，减少尿酸的产生。此外,SC能够增加肾脏和回肠中乳腺癌耐药蛋白（BCRP）的表达并增加尿酸的排泄。因此,我们的结果表明SC可能是抗高尿酸血症的候选药物.
