Abstract：
Pioneering approaches for precise tumor removal involve fluorescence-guided surgery, while challenges persist, including the low fluorescence contrast observed at tumor boundaries and the potential for excessive damage to normal tissue at the edges. Lead/cadmium sulfide quantum dots (PbS@CdS QDs), boasting high quantum yields (QYs) and vivid fluorescence, have facilitated advancements in the second near-infrared window (NIR-II, 900-1700 nm). However, during fluorescent surgical navigation operations, hydrophilic coatings of these inorganic nanoparticles (NPs) guarantee biosafety; it also comes at the expense of losing a significant portion of QY and NIR-II fluorescence, causing heightened damage to normal tissues caused by cutting edges. Herein, we present hydrophilic core-shell PbS@CdS@PEG NPs with an exceptionally small diameter (∼8 nm) and a brilliant NIR-IIb (1500-1700 nm) emission at approximately 1600 nm. The mPEG-SH (MW: 2000) addresses the hydrophobicity and enhances the biosafety of PbS@CdS QDs. In vivo fluorescence-guided cervical tumor resection becomes achievable immediately upon injection of an aqueous solution of PbS@CdS@PEG NPs. Notably, this approach results in a significantly reduced thickness (100-500 μm) of damage to normal tissues at the margins of the resected tumors. With a high QY (∼30.2%) and robust resistance to photobleaching, NIR-IIb imaging is sustained throughout the imaging process.
摘要：
精确切除肿瘤的开创性方法涉及荧光引导手术，虽然挑战依然存在，包括在肿瘤边界处观察到的低荧光对比度和对边缘处的正常组织的过度损伤的可能性。铅/硫化镉量子点(PbS@CdS量子点),拥有高量子产率（QYs）和生动的荧光，促进了第二个近红外窗口(NIR-II，900-1700nm）。然而,在荧光手术导航操作期间，这些无机纳米粒子（NPs）的亲水涂层保证了生物安全性;它也是以损失QY和NIR-II荧光的显著部分为代价的，对正常组织造成的高度损伤。在这里，我们提出了亲水的核-壳PbS@CdS@PEGNP，其直径异常小（〜8nm）和明亮的NIR-IIb（1500-1700nm）发射约1600nm。mPEG-SH（MW：2000）解决了PbS@CdSQD的疏水性并增强了其生物安全性。体内荧光引导的宫颈肿瘤切除术在注射PbS@CdS@PEGNP的水溶液后立即可实现。值得注意的是,这种方法导致对切除肿瘤边缘正常组织的损伤厚度显著减小(100-500μm).具有高QY（〜30.2%）和强大的抗光漂白能力，NIR-IIb成像在整个成像过程中持续。
