Abstract：
Full-thickness skin defects often are managed with split-thickness skin grafting. The wound healing process, including formation of new vessels during the healing of skin grafts, is complex.
To evaluate the microcirculatory changes in the treated tissue after skin grafting to analyze perfusion dynamics during the wound healing process.
Fourteen full-thickness skin defects were created on the back of 14 adult male Lewis rats. All wounds were treated with autologous split-thickness skin grafts. The perfusion dynamics were assessed for 84 days with an O2C device that combines a laser light to determine blood flow and white light to determine postcapillary SO2 and the rHb.
Blood flow increased for 50 days after grafting. SO2 decreased in superficial skin layers (depth of 2 mm) and increased in deep skin layers (depth of 8 mm) during the entire observation period. The rHb increased until day 10 in superficial layers and until day 20 in deep tissue layers.
The microcirculatory changes reflect the different phases of wound healing. Long after the skin transplants were macroscopically healed, alterations in microcirculation were still detected. These alterations were caused by the long-lasting changes in tissue metabolism due to the formation, conversion, and degradation of the dermal matrix and vessels during wound healing and scar formation.
To evaluate the microcirculatory changes in the treated tissue after skin grafting to analyze perfusion dynamics during the wound healing process.
Fourteen full-thickness skin defects were created on the back of 14 adult male Lewis rats. All wounds were treated with autologous split-thickness skin grafts. The perfusion dynamics were assessed for 84 days with an O2C device that combines a laser light to determine blood flow and white light to determine postcapillary SO2 and the rHb.
Blood flow increased for 50 days after grafting. SO2 decreased in superficial skin layers (depth of 2 mm) and increased in deep skin layers (depth of 8 mm) during the entire observation period. The rHb increased until day 10 in superficial layers and until day 20 in deep tissue layers.
The microcirculatory changes reflect the different phases of wound healing. Long after the skin transplants were macroscopically healed, alterations in microcirculation were still detected. These alterations were caused by the long-lasting changes in tissue metabolism due to the formation, conversion, and degradation of the dermal matrix and vessels during wound healing and scar formation.
摘要：
背景：全厚度皮肤缺损通常通过分层厚度皮肤移植来治疗。伤口愈合过程，包括在植皮愈合过程中形成新的血管，是复杂的。
目的：评估皮肤移植后治疗组织的微循环变化，以分析伤口愈合过程中的灌注动力学。
方法：在14只成年雄性Lewis大鼠的背部制造了14个全层皮肤缺损。所有伤口均用自体分层厚度皮肤移植物治疗。使用O2C设备评估灌注动力学84天，该设备结合了激光来确定血流量和白光来确定毛细血管后SO2和rHb。
结果：移植后50天血流量增加。在整个观察期间，SO2在浅皮肤层（深度为2mm）中减少，而在深皮肤层（深度为8mm）中增加。rHb在浅层中增加直到第10天,在深组织层中增加直到第20天。
结论：微循环变化反映了伤口愈合的不同阶段。在皮肤移植被宏观治愈后很久，仍检测到微循环的改变.这些改变是由组织代谢的持久变化引起的，转换，以及在伤口愈合和瘢痕形成期间真皮基质和血管的降解。
目的：评估皮肤移植后治疗组织的微循环变化，以分析伤口愈合过程中的灌注动力学。
方法：在14只成年雄性Lewis大鼠的背部制造了14个全层皮肤缺损。所有伤口均用自体分层厚度皮肤移植物治疗。使用O2C设备评估灌注动力学84天，该设备结合了激光来确定血流量和白光来确定毛细血管后SO2和rHb。
结果：移植后50天血流量增加。在整个观察期间，SO2在浅皮肤层（深度为2mm）中减少，而在深皮肤层（深度为8mm）中增加。rHb在浅层中增加直到第10天,在深组织层中增加直到第20天。
结论：微循环变化反映了伤口愈合的不同阶段。在皮肤移植被宏观治愈后很久，仍检测到微循环的改变.这些改变是由组织代谢的持久变化引起的，转换，以及在伤口愈合和瘢痕形成期间真皮基质和血管的降解。
