Abstract：
In this study, EPS production conditions of Geobacillus thermodenitrificans HBB 111, a thermophilic microorganism, were optimized and the amount of produced EPS (EPS 111) was found to be 44.0 mg/L. EPS 111 was purified using ion exchange chromatography and gel filtration chromatography, and a single type of exopolysaccharide was obtained. The structure of the purified EPS 111 was evaluated by TLC, FTIR, NMR, and GC-MS, and it was observed that it contained hexose (glucose, fructose, galactose and mannose) and pentose sugars. From the SEM photographs, it was understood that EPS 111 had an amorphous, rough, and layered structure. It was found that purified EPS 111 had low cytotoxicity (2.3%) and exhibited high antioxidant activity and remarkable antidiabetic, prebiotic and fibrinolytic activities. It is very valuable that the purified EPS 111 in this study offers multiple biological activities compared to the thermophilic EPSs reported in the literature and has a high potential for use in biotechnological and biomedical fields.
摘要：
在这项研究中,嗜热微生物HBB111的产EPS条件,进行了优化，发现产生的EPS（EPS111）的量为44.0mg/L。EPS111使用离子交换色谱和凝胶过滤色谱纯化，并获得单一类型的胞外多糖。通过TLC评估纯化的EPS111的结构，FTIR,NMR,和GC-MS，观察到它含有己糖(葡萄糖，果糖,半乳糖和甘露糖）和戊糖。从SEM照片，据了解，EPS111具有无定形，粗糙，和分层结构。发现纯化的EPS111具有较低的细胞毒性(2.3%),表现出较高的抗氧化活性和显著的抗糖尿病作用,益生元和纤溶活性。与文献中报道的嗜热EPS相比，本研究中纯化的EPS111提供了多种生物活性，并且在生物技术和生物医学领域具有很高的应用潜力。
