Abstract：
Besides the historical and traditional use of nuclear magnetic resonance (NMR) spectroscopy as a structure elucidation tool for proteins and metabolites, its quantification ability allows the determination of metabolite amounts and therefore enzymatic activity measurements. For this purpose, 1H-NMR with adapted water pulse pre-saturation sequences and calibration curves with commercial standard solutions can be used to quantify the photorespiratory cycle intermediates, 2-phosphoglycolate and glycolate, associated with the phosphoglycolate phosphatase reaction. The intensity of the 1H-NMR signal of glycolate produced by the activity of purified recombinant Arabidopsis thaliana PGLP1 can therefore be used to determine PGLP1 enzymatic activities and kinetic parameters.
摘要：
除了历史和传统使用核磁共振（NMR）光谱作为蛋白质和代谢物的结构阐明工具，其定量能力允许代谢物量的测定，因此酶活性的测量。为此,具有适应的水脉冲预饱和序列的1H-NMR和具有商业标准溶液的校准曲线可用于量化光呼吸循环中间体，2-磷酸乙醇酸盐和乙醇酸盐，与磷酸乙酸磷酸酶反应有关。因此,由纯化的重组拟南芥PGLP1的活性产生的乙醇酸盐的1H-NMR信号强度可用于测定PGLP1酶活性和动力学参数。
