PDF(Pubmed)
Abstract：
The increasing applications for eicosapentaenoic acid (EPA) and the potential shortfall in supply due to sustainability and contamination issues related with its conventional sources (i.e., fish oils; seafood) led to an extensive search for alternative and sustainable sources, as well as production processes. The present mini-review covers all the steps involved in the production of EPA from microorganisms, with a deeper focus on microalgae. From production systems to downstream processing, the most important achievements within each area are briefly highlighted. Comparative tables of methodologies are also provided, as well as additional references of recent reviews, so that readers may deepen their knowledge in the different issues addressed. KEY POINTS: • Microorganisms are more sustainable alternative sources of EPA than fish. • Due to the costly separation from DHA, species that produce only EPA are preferable. • EPA production can be optimised using non-genetic and genetic tailoring engineering.
摘要：
二十碳五烯酸（EPA）的应用日益增加，并且由于其常规来源的可持续性和污染问题而导致的潜在供应短缺（即，鱼油；海鲜)导致广泛寻找替代和可持续来源，以及生产工艺。本小型审查涵盖了从微生物生产EPA的所有步骤，更专注于微藻。从生产系统到下游加工，简要强调了每个领域最重要的成就。还提供了方法的比较表，以及最近评论的其他参考资料，以便读者可以加深他们对所解决的不同问题的了解。要点：•微生物是比鱼类更可持续的EPA替代来源。•由于从DHA中分离的成本很高，只产生EPA的物种是优选的。•EPA生产可以使用非遗传和遗传剪裁工程进行优化。
