PDF(Pubmed)
Abstract：
Rad6 functions as a ubiquitin-conjugating protein that regulates cellular processes in many fungal species. However, its role in filamentous entomopathogenic fungi remains poorly understood. This study characterizes Rad6 in Beauveria bassiana, a filamentous fungus widely employed as a critical fungicide globally. The results demonstrate a significant association between Rad6 and conidial properties, heat shock response, and UV-B tolerance. Concurrently, the mutant strain exhibited heightened sensitivity to oxidative stress, cell wall interfering agents, DNA damage stress, and prolonged heat shock. Furthermore, the absence of Rad6 significantly extended the median lethal time (LT50) of Galleria mellonella infected by B. bassiana. This delay could be attributed to reduced Pr1 proteases and extracellular cuticle-degrading enzymes, diminished dimorphic transition rates, and dysregulated antioxidant enzymes. Additionally, the absence of Rad6 had a more pronounced effect on genetic information processing, metabolism, and cellular processes under normal conditions. However, its impact was limited to metabolism in oxidative stress. This study offers a comprehensive understanding of the pivotal roles of Rad6 in conidial and hyphal stress tolerance, environmental adaptation, and the pathogenesis of Beauveria bassiana.
摘要：
Rad6在许多真菌物种中作为调节细胞过程的泛素结合蛋白起作用。然而,它在丝状昆虫病原真菌中的作用仍然知之甚少。这项研究描述了白僵菌中Rad6的特征，一种丝状真菌，在全球范围内被广泛用作关键的杀真菌剂。结果表明Rad6与分生孢子特性之间存在显着关联，热冲击响应，和UV-B耐受性。同时,突变菌株对氧化应激表现出更高的敏感性，细胞壁干扰剂，DNA损伤应激,和长时间的热休克。此外，Rad6的缺失显着延长了由B.basiana感染的GalleriaMellonella的中位致死时间（LT50）。这种延迟可能归因于减少的Pr1蛋白酶和细胞外表皮降解酶，二态过渡率降低，和抗氧化酶失调。此外,Rad6的缺失对基因信息处理有更明显的影响，新陈代谢，和正常条件下的细胞过程。然而,其影响仅限于氧化应激代谢。这项研究提供了一个全面的了解Rad6在分生孢子和菌丝应激耐受性的关键作用，环境适应,球孢白僵菌的发病机制。
