PDF(Pubmed)
Abstract：
Colorectal cancer (CRC) is among the most prevalent malignant tumors, known for its high heterogeneity. Although many treatments and medications are available, the long-term survival rate of CRC patients is far from satisfactory. Pyroptosis is closely related to tumor progression. This study aimed to identify pyroptosis-related genes (PRGs) and candidate biomarkers to predict the prognosis of CRC patients. Used bioinformatics, we identified PRGs and subsequently screened 288 co-expression genes between pyroptosis-related modules and differentially expressed genes in CRC. Among these hub genes, we selected the top 24 for further analysis and found that Radical S-Adenosyl Methionine Domain Containing 2 (RSAD2) was a novel biomarker associated with the progression of CRC. We developed a risk model for RSAD2, which proved to be an independent prognostic indicator. The receiver operator characteristic analysis showed that the model had an acceptable prognostic value for patients with CRC. In addition, RSAD2 also affects the tumor immune microenvironment and prognosis of CRC. We further validated RSAD2 expression in CRC patients using RT-qPCR and the role of RSAD2 in pyroptosis. Taken together, this study comprehensively assessed the expression and prognostic value of RSAD2 in patients with CRC. These findings may offer a new direction for early CRC screening and development of future immunotherapy strategies.
摘要：
结直肠癌（CRC）是最常见的恶性肿瘤之一，以其高度异质性而闻名。虽然有许多治疗方法和药物，CRC患者的长期生存率远不能令人满意。发热与肿瘤进展密切相关。本研究旨在鉴定焦亡相关基因（PRGs）和候选生物标志物，以预测CRC患者的预后。使用生物信息学，我们鉴定了PRGs,随后筛选了CRC中与焦凋亡相关模块和差异表达基因之间的288个共表达基因.在这些中枢基因中，我们选择了前24名进行进一步分析,发现自由基S-腺苷蛋氨酸结构域包含2(RSAD2)是与CRC进展相关的新型生物标志物.我们开发了RSAD2的风险模型，该模型被证明是独立的预后指标。接收器操作员特征分析表明，该模型对CRC患者具有可接受的预后价值。此外,RSAD2还影响CRC的肿瘤免疫微环境和预后。我们使用RT-qPCR进一步验证了RSAD2在CRC患者中的表达以及RSAD2在焦亡中的作用。一起来看,本研究全面评估了RSAD2在CRC患者中的表达和预后价值。这些发现可能为早期CRC筛查和未来免疫治疗策略的发展提供新的方向。
