PDF(Pubmed)
Abstract：
T lymphocyte activation plays a pivotal role in adaptive immune response and alters the spatial organization of nuclear architecture that subsequently impacts transcription activities. Here, using stochastic optical reconstruction microscopy (STORM), we observe dramatic de-condensation of chromatin and the disruption of nuclear envelope at a nanoscale resolution upon T lymphocyte activation. Super-resolution imaging reveals that such alterations in nuclear architecture are accompanied by the release of nuclear DNA into the cytoplasm, correlating with the degree of chromatin decompaction within the nucleus. The authors show that under the influence of metabolism, T lymphocyte activation de-condenses chromatin, disrupts the nuclear envelope, and releases DNA into the cytoplasm. Taken together, this result provides a direct, molecular-scale insight into the alteration in nuclear architecture. It suggests the release of nuclear DNA into the cytoplasm as a general consequence of chromatin decompaction after lymphocyte activation.
摘要：
T淋巴细胞活化在适应性免疫反应中起关键作用，并改变核结构的空间组织，从而影响转录活性。这里,使用随机光学重建显微镜(STORM)，我们观察到T淋巴细胞激活后，染色质的急剧凝结和核膜的纳米级破坏。超分辨率成像显示，核结构的这种改变伴随着核DNA释放到细胞质中，与细胞核内染色质分解的程度有关。作者表明，在新陈代谢的影响下，T淋巴细胞活化去缩合染色质，破坏了核外壳，并将DNA释放到细胞质中。一起来看,这个结果提供了一个直接的，分子尺度洞察核结构的变化。这表明淋巴细胞活化后染色质分解的一般结果是核DNA释放到细胞质中。
