Abstract：
Gut microbiota dysbiosis increases the susceptibility to Clostridioides difficile infection (CDI). In this study, we monitored C. difficile colonization (CDC) patients from no CDC status (CDN) to CDC status (CDCp) and CDI patients from asymptomatic status before CDI (PRECDI), CDI status (ONCDI), to asymptomatic status after CDI (POSTCDI). Based on metagenomic sequencing, we aimed to investigate the interaction pattern between gut microbiota and C. difficile. There was no significant difference of microbiota diversity between CDN and CDCp. In CDCp, Bacteroidetes and short-chain fatty acid (SCFA)-producing bacteria increased, with a positive correlation between SCFA-producing bacteria and C. difficile colonization. Compared with PRECDI, ONCDI and POSTCDI showed a significant decrease in microbiota diversity, particularly in Bacteroidetes and SCFA-producing bacteria, with a positive correlation between opportunistic pathogen and C. difficile. Fatty acid metabolism, and amino acid biosynthesis were enriched in CDN, CDCp, and PRECDI, while bile secretion was enriched in ONCDI and POSTCDI. Microbiota and metabolic pathways interaction networks in CDN and CDCp were more complex, particularly pathways in fatty acid and bile acid metabolism. Increasing of Bacteroidetes and SCFA-producing bacteria, affecting amino acid and fatty acid metabolism, is associated with colonization resistance to C. difficile and inhibiting the development of CDI.
摘要：
肠道菌群失调增加了艰难梭菌感染（CDI）的易感性。在这项研究中,我们监测艰难梭菌定植（CDC）患者从无CDC状态（CDN）到CDC状态（CDCp），CDI患者从CDI前无症状状态（PRECDI），CDI状态(ONCDI),CDI后无症状状态（后CDI）。基于宏基因组测序，我们旨在研究肠道菌群与艰难梭菌之间的相互作用模式。CDN和CDCp之间的微生物群多样性没有显着差异。在CDCP中,拟杆菌和产生短链脂肪酸（SCFA）的细菌增加，SCFA产生菌与艰难梭菌定植呈正相关。与PRECDI相比，ONCDI和POSTCDI显示微生物群多样性显著下降，特别是在拟杆菌和产生SCFA的细菌中，机会致病菌与艰难梭菌呈正相关。脂肪酸代谢，氨基酸生物合成富集在CDN中，CDCP,PRECDI,而胆汁分泌在ONCDI和POSTCDI中富集。CDN和CDCp中的微生物群和代谢途径相互作用网络更为复杂，特别是脂肪酸和胆汁酸代谢途径。拟杆菌和产生SCFA的细菌的增加，影响氨基酸和脂肪酸代谢，与对艰难梭菌的定植抗性和抑制CDI的发展有关。
