Abstract：
Selenium and selenoproteins are closely related to melanoma progression. However, it is unclear how SELENOK affects lipid metabolism, endoplasmic reticulum stress (ERS), immune cell infiltration, survival, and prognosis in melanoma patients. Transcriptome data from melanoma patients was used to investigate SELENOK levels and their effect on prognosis, followed by an investigation of SELENOK\'s effects on immune cell infiltration. Furthermore, a risk model based on ERS, lipid metabolism, and immune-related genes was constructed, and its utility in melanoma prognosis was evaluated. Finally, the drug sensitivity of the risk model was analyzed to provide a reference for melanoma therapy. The results showed that melanoma with a high SELENOK level had a greater degree of immune cell infiltration and a better prognosis. Additionally, SELENOK was found to regulate ERS, lipid metabolism, and immune cell infiltration in melanoma. The risk model based on SELENOK signature genes successfully predicted the prognosis of melanoma, and the low-risk group exhibited a favorable immunological microenvironment. Furthermore, high-risk patients with melanoma were candidates for chemotherapy with RAS pathway inhibitors, whereas low-risk patients were more susceptible to routinely used chemotherapy medicines. In summary, SELENOK was shown to regulate ERS, lipid metabolism, and immune cell infiltration in melanoma, and SELENOK was positively associated with the prognosis of melanoma. The risk model based on SELENOK signature genes was valuable for melanoma prognosis and therapy.
摘要：
硒和硒蛋白与黑色素瘤的进展密切相关。然而,尚不清楚SELENOK如何影响脂质代谢，内质网应激（ERS），免疫细胞浸润，生存,黑色素瘤患者的预后。来自黑色素瘤患者的转录组数据用于研究SELENOK水平及其对预后的影响，随后研究了SELENOK对免疫细胞浸润的影响。此外，基于ERS的风险模型，脂质代谢，并构建了免疫相关基因，并评估了其在黑色素瘤预后中的实用性。最后,分析风险模型的药物敏感性,为黑色素瘤的治疗提供参考。结果表明，SELENOK水平较高的黑色素瘤具有较大程度的免疫细胞浸润，预后较好。此外,SELENOK被发现监管ERS,脂质代谢，和黑色素瘤中的免疫细胞浸润。基于SELENOK特征基因的风险模型成功预测黑色素瘤的预后,低危组表现出良好的免疫微环境。此外，高危黑色素瘤患者是RAS途径抑制剂化疗的候选人，而低危患者对常规化疗药物更敏感.总之,SELENOK被证明可以调节ERS，脂质代谢，和黑色素瘤中的免疫细胞浸润，SELENOK与黑色素瘤的预后呈正相关。基于SELENOK特征基因的风险模型对黑色素瘤的预后和治疗有价值。
