PDF(Pubmed)
Abstract：
Plasmodiophora brassicae (Woronin, 1877), a biotrophic, obligate parasite, is the causal agent of clubroot disease in brassicas. The clubroot pathogen has been reported in more than 80 countries worldwide, causing economic losses of hundreds of millions every year. Despite its widespread impact, very little is known about the molecular strategies it employs to induce the characteristic clubs in the roots of susceptible hosts during infection, nor about the mechanisms it uses to overcome genetic resistance. Here, we provide the first telomere-to-telomere complete genome of P. brassicae. We generated ∼27 Gb of Illumina, Oxford Nanopore, and PacBio HiFi data from resting spores of strain Pb3A and produced a 25.3 Mb assembly comprising 20 chromosomes, with an N50 of 1.37 Mb. The BUSCO score, the highest reported for any member of the group Rhizaria (Eukaryota: 88.2%), highlights the limitations within the Eukaryota database for members of this lineage. Using available transcriptomic data and protein evidence, we annotated the Pb3A genome, identifying 10,521 protein-coding gene models. This high-quality, complete genome of P. brassicae will serve as a crucial resource for the plant pathology community to advance the much-needed understanding of the evolution of the clubroot pathogen.
摘要：
油菜疟原虫(沃罗宁，1877),一个生物营养学家，专性寄生虫,是油菜根肿病的病因。在全球80多个国家已经报道了根茎病病原体，每年造成数以亿计的经济损失。尽管影响广泛,关于它在感染期间在易感宿主的根部诱导特征性俱乐部的分子策略知之甚少，也不知道它用来克服遗传抗性的机制。这里,我们提供了油菜疟原虫的第一个端粒至端粒完整基因组。我们产生了27Gb的Illumina，牛津纳米孔,和PacBioHiFi数据来自Pb3A菌株的静止孢子，并产生了包含20条染色体的25.3Mb组件，N50为1.37Mb。BUSCO得分,Rhizaria组中任何成员的最高报告（Eukaryota：88.2％），强调了Eukaryota数据库中针对该谱系成员的限制。利用现有的转录组数据和蛋白质证据，我们注释了Pb3A基因组，鉴定10521个蛋白质编码基因模型。这种高品质，油菜疟原虫的完整基因组将成为植物病理学界的重要资源，以促进对根茎病原体进化的急需的理解。
