PDF(Pubmed)
Abstract：
Polypyrrole (Ppy) is a biologically compatible polymer that is used as a matrix, in which drugs and enzymes can be incorporated by doping. Here, we suggest an inventive application of Ppy as a biorecognition film encapsulated with an antibody (Ab) as an alternative strategy for the on-site multistep functionalization of thiol-based self-assembled monolayers. The fabrication steps of the recognition films were followed by dropping pyrrole and Ab mixed solutions onto the electrode and obtaining a thin film by direct current electropolymerization. The efficiency of Ab immobilization was studied by using fluorescence microscopy and electrochemical (EC) methods. Finally, the Ab density was increased and immobilized in 1 min, and the sensing performance as an EC immunosensor was demonstrated using α-fetoprotein with a limit of detection of 3.13 pg/mL and sensing range from 1 pg/mL to 100 ng/mL. This study demonstrates the potential for electrochemical functionalization of biomolecules with high affinity and rapidity.
摘要：
聚吡咯（Ppy）是一种生物相容性聚合物，用作基质，其中药物和酶可以通过掺杂掺入。这里,我们建议Ppy作为用抗体（Ab）封装的生物识别膜的创造性应用，作为基于硫醇的自组装单层的现场多步官能化的替代策略。在识别膜的制造步骤之后,将吡咯和Ab混合溶液滴到电极上,并通过直流电聚合获得薄膜。通过使用荧光显微镜和电化学（EC）方法研究了Ab固定的效率。最后,Ab密度增加并在1分钟内固定，使用α-甲胎蛋白证明了作为EC免疫传感器的传感性能，检测限为3.13pg/mL，传感范围为1pg/mL至100ng/mL。这项研究证明了具有高亲和力和快速性的生物分子的电化学官能化的潜力。
