Abstract：
Reducing monosaccharides and their phosphates are critical metabolites in the central carbon metabolism pathway of living organisms. Variations in their content can indicate abnormalities in metabolic pathways and the onset of certain diseases, necessitating their analysis and detection. Reducing monosaccharides and their phosphates exhibit significant variations in content within biological samples and are present in many isomers, which makes the accurate quantification of reducing monosaccharides and their phosphates in biological samples a challenging task. Various analytical methods such as spectroscopy, fluorescence detection, colorimetry, nuclear magnetic resonance spectroscopy, sensor-based techniques, chromatography, and mass spectrometry are employed to detect monosaccharides and phosphates. In comparison, chromatography and mass spectrometry are highly favored for their ability to simultaneously analyze multiple components and their high sensitivity and selectivity. This review thoroughly evaluates the current chromatographic and mass spectrometric methods used for detecting reducing monosaccharides and their phosphates from 2013 to 2023, highlighting their efficacy and the advancements in these analytical technologies.
摘要：
还原单糖及其磷酸盐是生物体中央碳代谢途径中的关键代谢产物。其含量的变化可以表明代谢途径的异常和某些疾病的发作，需要他们的分析和检测。还原单糖及其磷酸盐在生物样品中的含量存在显着变化，并且存在于许多异构体中，这使得对生物样品中还原单糖及其磷酸盐的准确定量成为一项具有挑战性的任务。各种分析方法，如光谱学，荧光检测,比色法,核磁共振波谱,基于传感器的技术，色谱，和质谱法用于检测单糖和磷酸盐。相比之下,色谱和质谱因其同时分析多种成分的能力以及高灵敏度和选择性而备受青睐。这篇综述全面评估了2013年至2023年用于检测还原单糖及其磷酸盐的色谱和质谱方法，突出了它们的功效和这些分析技术的进步。
