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Abstract：
UNASSIGNED: The presence of fungi in the respiratory tract as mycobiome, particularly Candida species (spp.), remains a serious problem due to increasing numbers of immunocompromised patients. The confirmed reliable existence of these pathogens due to frequent colonization is essential. This investigation aimed to recognize Candida spp. among isolates from bronchoalveolar lavage of immunocompromised and critically ill patients and to evaluate their susceptibility to antimycotic drugs.
UNASSIGNED: Bronchoalveolar lavage fluid was collected from 161 hospitalized patients presenting with suspected respiratory fungal infection /colonization. The specimens were examined by standard molecular and mycological assays. Candida spp. were recognized with sequence assessment of the D1-D2 section of the large subunit ribosomal DNA. The susceptibility of Candida isolates to common antimycotic drugs was distinguished by standard broth microdilution.
UNASSIGNED: Seventy-one clinical isolates of Candida spp. were recognized. Candida albicans was the most frequent, followed by C. glabrata, C. krusei (Pichia kudriavzevii), C. dubliniensis, C. parapsilosis, and C. tropicalis. We found 5.1% of C. albicans isolates and 8% of C. glabrata isolates to show resistance to fluconazole. The whole of the Candida spp. were sensitive to amphotericin B and caspofungin.
UNASSIGNED: This study demonstrated that C. albicans and C. glabrata are the most common isolates of bronchoalveolar lavage fluid in patients, and the drug susceptibility screening confirmed that amphotericin B and caspofungin are effective against Candida spp. but some C. glabrata and C. albicans isolates showed resistance to fluconazole.
UNASSIGNED: Bronchoalveolar lavage fluid was collected from 161 hospitalized patients presenting with suspected respiratory fungal infection /colonization. The specimens were examined by standard molecular and mycological assays. Candida spp. were recognized with sequence assessment of the D1-D2 section of the large subunit ribosomal DNA. The susceptibility of Candida isolates to common antimycotic drugs was distinguished by standard broth microdilution.
UNASSIGNED: Seventy-one clinical isolates of Candida spp. were recognized. Candida albicans was the most frequent, followed by C. glabrata, C. krusei (Pichia kudriavzevii), C. dubliniensis, C. parapsilosis, and C. tropicalis. We found 5.1% of C. albicans isolates and 8% of C. glabrata isolates to show resistance to fluconazole. The whole of the Candida spp. were sensitive to amphotericin B and caspofungin.
UNASSIGNED: This study demonstrated that C. albicans and C. glabrata are the most common isolates of bronchoalveolar lavage fluid in patients, and the drug susceptibility screening confirmed that amphotericin B and caspofungin are effective against Candida spp. but some C. glabrata and C. albicans isolates showed resistance to fluconazole.
摘要：
■呼吸道中存在真菌，特别是念珠菌属（spp。),仍然是一个严重的问题，由于越来越多的免疫功能低下的患者。由于频繁的定植，这些病原体的可靠存在是必不可少的。这项调查旨在识别念珠菌属。在免疫功能低下和危重患者的支气管肺泡灌洗分离株中，并评估其对抗真菌药物的敏感性。
■从161例疑似呼吸道真菌感染/定植的住院患者中收集支气管肺泡灌洗液。通过标准分子和真菌学测定检查样本。念珠菌属。通过对大亚基核糖体DNA的D1-D2部分的序列评估来识别。通过标准肉汤微量稀释来区分念珠菌分离株对常见抗真菌药物的敏感性。
■71种念珠菌临床分离株。被认可。白色念珠菌是最常见的，其次是C.glabrata,C.krusei(Pichiakudriavzevii),都柏林人，C.近平滑，和热带C.我们发现5.1％的白色念珠菌分离株和8％的光滑念珠菌分离株显示出对氟康唑的抗性。整个念珠菌属。对两性霉素B和卡泊芬净敏感。
■这项研究表明，白色念珠菌和光滑念珠菌是患者支气管肺泡灌洗液中最常见的分离株，药物敏感性筛选证实两性霉素B和卡泊芬净对念珠菌有效。但是一些光滑念珠菌和白色念珠菌分离株对氟康唑表现出耐药性。
■从161例疑似呼吸道真菌感染/定植的住院患者中收集支气管肺泡灌洗液。通过标准分子和真菌学测定检查样本。念珠菌属。通过对大亚基核糖体DNA的D1-D2部分的序列评估来识别。通过标准肉汤微量稀释来区分念珠菌分离株对常见抗真菌药物的敏感性。
■71种念珠菌临床分离株。被认可。白色念珠菌是最常见的，其次是C.glabrata,C.krusei(Pichiakudriavzevii),都柏林人，C.近平滑，和热带C.我们发现5.1％的白色念珠菌分离株和8％的光滑念珠菌分离株显示出对氟康唑的抗性。整个念珠菌属。对两性霉素B和卡泊芬净敏感。
■这项研究表明，白色念珠菌和光滑念珠菌是患者支气管肺泡灌洗液中最常见的分离株，药物敏感性筛选证实两性霉素B和卡泊芬净对念珠菌有效。但是一些光滑念珠菌和白色念珠菌分离株对氟康唑表现出耐药性。
