UNASSIGNED: We identified the relationship between GPRC5A expression and the clinicopathologic characteristics of NSCLC patients in the Fudan University Shanghai Cancer Center (FUSCC) cohort. Furthermore, we validated GPRC5A as a predictive biomarker by using public databases to reveal the relationship between GPRC5A expression and immune cell infiltration. To correlate the expression of GPRC5A with the spatial distribution of PD-L1 in NSCLC samples, we performed multiplex immunohistochemistry (mIHC).
UNASSIGNED: Low GPRC5A expression is associated with earlier pathological stage (pStage). Analysis of immune cell infiltration indicates there is a relationship between low GPRC5A expression and increased infiltration of CD8+ T cells, activated CD4+ T cells, and M1 macrophages within the TME. Furthermore, low GPRC5A expression is associated with an increased immunophenotype score (IPS) in NSCLC. Additionally, analysis of mIHC reveals there is a correlation between low GPRC5A expression and spatial distribution of tumoral PD-L1 expression.
UNASSIGNED: Our study revealed the relationship between low expression of GPRC5A and earlier pStage in NSCLC. Furthermore, we observed that low expression of GPRC5A is associated with increased infiltration of immune cells, higher IPS, and spatial distribution of PD-L1-positive tumor cells. Therefore, we speculate that low expression of GPRC5A is associated with immunotherapy, but further validation is still required.
■我们在复旦大学上海肿瘤防治中心(FUSCC)队列中确定GPRC5A表达与NSCLC患者临床病理特征之间的关系。此外,我们通过使用公共数据库验证GPRC5A作为预测生物标志物,以揭示GPRC5A表达与免疫细胞浸润之间的关系.为了将GPRC5A的表达与NSCLC样本中PD-L1的空间分布相关联,我们进行了多重免疫组织化学(mIHC)。
■低GPRC5A表达与早期病理阶段(pStage)相关。免疫细胞浸润分析表明,GPRC5A低表达与CD8+T细胞浸润增加之间存在关系,激活的CD4+T细胞,和TME内的M1巨噬细胞。此外,GPRC5A低表达与NSCLC中免疫表型评分(IPS)升高相关.此外,mIHC分析显示GPRC5A低表达与肿瘤PD-L1表达的空间分布之间存在相关性。
■我们的研究揭示了GPRC5A的低表达与NSCLC早期pStage之间的关系。此外,我们观察到GPRC5A的低表达与免疫细胞浸润增加有关,较高的IPS,PD-L1阳性肿瘤细胞的空间分布。因此,我们推测GPRC5A的低表达与免疫治疗有关,但仍需要进一步验证。